Casimiro-Soriguer, Carlos S.Perez-Florido, JavierFernandez-Rueda, Jose L.Pedrosa-Corral, IreneGuillot-Sulay, VicenteLorusso, NicolaMartinez-Gonzalez, Luis JavierNavarro-Marí, Jose M.Dopazo, JoaquinSanbonmatsu-Gámez, Sara2022-03-082022-03-082021-05-05Casimiro-Soriguer CS, Perez-Florido J, Fernandez-Rueda JL, Pedrosa-Corral I, Guillot-Sulay V, Lorusso N, et al. Phylogenetic Analysis of the 2020 West Nile Virus (WNV) Outbreak in Andalusia (Spain). Viruses. 2021; 13(5):836.http://hdl.handle.net/10668/3467During recent decades West Nile Virus (WNV) outbreaks have continuously occurred in the Mediterranean area. In August 2020 a new WNV outbreak affected 71 people with meningoencephalitis in Andalusia and six more cases were detected in Extremadura (south-west of Spain), causing a total of eight deaths. The whole genomes of four viruses were obtained and phylogenetically analyzed in the context of recent outbreaks. The Andalusian viral samples belonged to lineage 1 and were relatively similar to those of previous outbreaks which occurred in the Mediterranean region. Here we present a detailed analysis of the outbreak, including an extensive phylogenetic study. As part on this effort, we implemented a local Nextstrain server, which has become a constituent piece of regional epidemiological surveillance, wherein forthcoming genomes of environmental samples or, eventually, future outbreaks, will be included.enAttribution-NonCommercial-NoDerivatives 4.0 InternacionalAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/West Nile virusOutbreakMeningoencephalitisEpidemiologyPhylogenyWhole genome sequencingFiebre del Nilo occidentalBrotes de enfermedadesMeningoencefalitisEpidemiologíaFilogeniaSecuenciación completa del genomaMedical Subject Headings::Health Care::Environment and Public Health::Public Health::Disease OutbreaksMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA::DNA Mutational AnalysisMedical Subject Headings::Geographical Locations::Geographic Locations::Europe::SpainMedical Subject Headings::Diseases::Nervous System Diseases::Central Nervous System Diseases::Central Nervous System Infections::Central Nervous System Viral Diseases::Encephalitis::Encephalitis, Viral::Encephalitis, Arbovirus::West Nile FeverMedical Subject Headings::Organisms::Viruses::Vertebrate Viruses::RNA Viruses::Encephalitis Viruses::Encephalitis Viruses, Japanese::West Nile virusMedical Subject Headings::Information Science::Information Science::Classification::Phylogenyresearch article34063166open access10.3390/v130508361999-4915PMC8148183