Aguirre-Quiñonero, ACano, M EGamal, DCalvo, JMartínez-Martínez, L2025-01-072025-01-072017-03-16https://hdl.handle.net/10668/26278The objective of this study was to evaluate the accuracy of the CIM test in the detection of carbapenemase activity in 124 strains of Enterobacteriaceae. A panel of 124 previously characterized Enterobacteriaceae was tested: 77 strains producing the following carbapenemase families: KPC (n = 14), GES (n = 22), NDM (n = 19), VIM (n = 4), IMP (n = 4) and OXA-48 (n = 14) and 47 non-carbapenemase producers. For the CIM method, an active susceptibility meropenem disc was exposed to a bacterial suspension of a test strain; when a carbapenemase is produced, the antibiotic is inactivated allowing uninhibited growth of an indicator strain after overnight incubation. A clear inhibition zone (≥20 mm) was considered indicative of no-carbapenemase activity. All KPC, NDM, VIM, IMP or OXA-48 producing strains were unequivocally detected with the CIM test. CIM false negative results were obtained with eleven Enterobacter cloacae producing GES-6. Two other E. cloacae not producing carbapenemase (one with SHV-12, one hyperproducing AmpC) were positive by the test. The sensitivity and specificity of the assay compared to those of molecular methods were 85.7% and 95.7%, respectively. The CIM method proved to be inexpensive and easy to interpret. It provided less than optimal results in the detection of GES-6 activity.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Carbapenem inactivation methodCarbapenemaseGES-6Rapid testAnti-Bacterial AgentsBacterial ProteinsBacteriological TechniquesCarbapenem-Resistant EnterobacteriaceaeCarbapenemsDiagnostic ErrorsHumansHydrolysisSensitivity and SpecificityTime Factorsbeta-Lactamasesresearch article28476242open access10.1016/j.diagmicrobio.2017.03.0091879-0070https://repositorio.unican.es/xmlui/bitstream/10902/11855/3/EvaluationCarbapenemInactivation.pdf