Perea, CristinaVázquez-Ágredos, AnaRuiz-Leyva, LeandroMorón, IgnacioZúñiga, Jesús MartínCendán, Cruz Miguel2022-11-212022-11-212021-04-15Perea C, Vázquez-Ágredos A, Ruiz-Leyva L, Morón I, Zúñiga JM, Cendán CM. Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data. Front Vet Sci. 2021 Apr 15;8:639187http://hdl.handle.net/10668/4383Much of the research done on aging, oxidative stress, anxiety, and cognitive and social behavior in rodents has focused on caloric restriction (CR). This often involves several days of single housing, which can cause numerous logistical problems, as well as cognitive and social dysfunctions. Previous results in our laboratory showed the viability of long-term CR in grouped rats. Our research has studied the possibility of CR in grouped female and male littermates and unrelated CB6F1/J (C57BL/6J × BALBc/J hybrid strain) mice, measuring: (i) possible differences in body mass proportions between mice in ad libitum and CR conditions (at 70% of ad libitum), (ii) aggressive behavior, using the number of pushes and chasing behavior time as an indicator and social behavior using the time under the feeder as indicator, and (iii) difference in serum adrenocorticotropic hormone (ACTH) concentrations (stress biomarker), under ad libitum and CR conditions. Results showed the impossibility of implementing CR in unrelated male mice. In all other groups, CR was possible, with a less aggressive behavior (measured only with the number of pushes) observed in the unrelated female mice under CR conditions. In that sense, the ACTH levels measured on the last day of CR showed no difference in stress levels. These results indicate that implementantion of long-term CR in mice can be optimized technically and also related to their well-being by grouping animals, in particular, related mice.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Caloric restrictionGrouped miceLittermate miceAdrenocorticotropicEating behaviorSocial behaviorAgingOxidative stressAnxietyRestricción calóricaHormona adrenocorticotrópicaConducta alimentariaConducta socialEnvejecimientoEstrés oxidativoAnsiedadMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::RatsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Nutrition Therapy::Diet Therapy::Caloric RestrictionMedical Subject Headings::Health Care::Population Characteristics::Demography::Residence Characteristics::HousingMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::AgingMedical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Social BehaviorMedical Subject Headings::Psychiatry and Psychology::Psychological Phenomena and Processes::Mental Processes::CognitionMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative StressMedical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pituitary Hormones::Pituitary Hormones, Anterior::Pro-Opiomelanocortin::Adrenocorticotropic HormoneMedical Subject Headings::Chemicals and Drugs::Biological Factors::Biological Markers::Biomarkers, PharmacologicalMedical Subject Headings::Psychiatry and Psychology::Behavior and Behavior Mechanisms::Emotions::Anxietyresearch article33937370Acceso abierto10.3389/fvets.2021.6391872297-1769PMC8081842