Castro-Castro, María JoséCandás-Estébanez, BeatrizEsteban-Salán, MargaritaCalmarza, PilarArrobas-Velilla, TeresaRomero-Román, CarlosPocoví-Mieras, MiguelAguilar-Doreste, José ÁngelCommission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica2023-01-252023-01-252018http://hdl.handle.net/10668/12730Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods-LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.enAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/1,1,2-trichlorotrifluoroethaneHigh-speed centrifugationInterferenceLipemiaLipid removal methodLipoClearAlanine TransaminaseCalciumCentrifugationCreatinineHumansHyperlipidemiasLaboratories, HospitalLipidsLiquid-Liquid Extractionresearch article30027694open access10.3343/alm.2018.38.6.5182234-3814PMC6056396http://www.annlabmed.org/journal/download_pdf.php?doi=10.3343/alm.2018.38.6.518https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056396/pdf