Teuwen, Laure-AnneDe Rooij, Laura P. M. H.Cuypers, AnneRohlenova, KaterinaDumas, Sébastien J.García-Caballero, MelissaMeta, EldaAmersfoort, JacobTaverna, FedericoBecker, Lisa M.Veiga, NupharCantelmo, Anna RitaGeldhof, VincentConchinha, Nadine V.Kalucka, JoannaTreps, LucasConradi, Lena-ChristinKhan, ShawezKarakach, Tobias K.Soenen, StefaanVinckier, StefanSchoonjans, LucEelen, GuyVan Laere, StevenDewerchin, MiekeDirix, LucMazzone, MassimilianoLuo, YonglunVermeulen, PeterCarmeliet, Peter2022-05-062022-05-062021-06-15Teuwen LA, De Rooij LPMH, Cuypers A, Rohlenova K, Dumas SJ, García-Caballero M, et al. Tumor vessel co-option probed by single-cell analysis. Cell Rep. 2021 Jun 15;35(11):109253.http://hdl.handle.net/10668/3614Tumor vessel co-option is poorly understood, yet it is a resistance mechanism against anti-angiogenic therapy (AAT). The heterogeneity of co-opted endothelial cells (ECs) and pericytes, co-opting cancer and myeloid cells in tumors growing via vessel co-option, has not been investigated at the single-cell level. Here, we use a murine AAT-resistant lung tumor model, in which VEGF-targeting induces vessel co-option for continued growth. Single-cell RNA sequencing (scRNA-seq) of 31,964 cells reveals, unexpectedly, a largely similar transcriptome of co-opted tumor ECs (TECs) and pericytes as their healthy counterparts. Notably, we identify cell types that might contribute to vessel co-option, i.e., an invasive cancer-cell subtype, possibly assisted by a matrix-remodeling macrophage population, and another M1-like macrophage subtype, possibly involved in keeping or rendering vascular cells quiescent.enAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Tumor vessel co-optionTumor angiogenesisAnti-angiogenic therapyMetastasisResistanceSingle-cell RNA sequencingEndothelial cellsPericytesMacrophagesCancer cellsInductores de la angiogénesisMetástasis de la neoplasiaCélulas endotelialesPericitosMacrófagosMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, TumorMedical Subject Headings::Anatomy::Cells::Epithelial Cells::Endothelial CellsMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Urologic Neoplasms::Kidney NeoplasmsMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Thoracic Neoplasms::Respiratory Tract Neoplasms::Lung NeoplasmsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Inbred Strains::Mice, Inbred BALB CMedical Subject Headings::Anatomy::Cells::Myeloid CellsMedical Subject Headings::Diseases::NeoplasmsMedical Subject Headings::Anatomy::Cells::PericytesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Single-Cell Analysisresearch article34133923Acceso abierto10.1016/j.celrep.2021.1092532211-1247