Travez, AndresRabanal-Ruiz, YoanaLopez-Alcala, JaimeMolero-Murillo, LauraDiaz-Ruiz, AlbertoGuzman-Ruiz, RocioCatalan, VictoriaRodriguez, AmaiaFrühbeck, GemaTinahones, Francisco JGasman, StephaneVitale, NicolasJimenez-Gomez, YolandaMalagon, Maria M2023-01-252023-01-252018-07-13Trávez A, Rabanal-Ruiz Y, López-Alcalá J, Molero-Murillo L, Díaz-Ruiz A, Guzmán-Ruiz R, et al. The caveolae-associated coiled-coil protein, NECC2, regulates insulin signalling in Adipocytes. J Cell Mol Med. 2018 Nov;22(11):5648-5661.http://hdl.handle.net/10668/12883Adipocyte dysfunction in obesity is commonly associated with impaired insulin signalling in adipocytes and insulin resistance. Insulin signalling has been associated with caveolae, which are coated by large complexes of caveolin and cavin proteins, along with proteins with membrane-binding and remodelling properties. Here, we analysed the regulation and function of a component of caveolae involved in growth factor signalling in neuroendocrine cells, neuroendocrine long coiled-coil protein-2 (NECC2), in adipocytes. Studies in 3T3-L1 cells showed that NECC2 expression increased during adipogenesis. Furthermore, NECC2 co-immunoprecipitated with caveolin-1 (CAV1) and exhibited a distribution pattern similar to that of the components of adipocyte caveolae, CAV1, Cavin1, the insulin receptor and cortical actin. Interestingly, NECC2 overexpression enhanced insulin-activated Akt phosphorylation, whereas NECC2 downregulation impaired insulin-induced phosphorylation of Akt and ERK2. Finally, an up-regulation of NECC2 in subcutaneous and omental adipose tissue was found in association with human obesity and insulin resistance. This effect was also observed in 3T3-L1 adipocytes exposed to hyperglycaemia/hyperinsulinemia. Overall, the present study identifies NECC2 as a component of adipocyte caveolae that is regulated in response to obesity and associated metabolic complications, and supports the contribution of this protein as a molecular scaffold modulating insulin signal transduction at these membrane microdomains.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/NECC2adipocytesadipogenesiscaveolaeinsulin resistanceinsulin signallingobesity3T3-L1 CellsAdipocytesAdipogenesisAdipose TissueAnimalsCaveolaeCaveolin 1HumansInsulinInsulin ResistanceMembrane ProteinsMiceMicrotubule-Associated ProteinsMitogen-Activated Protein Kinase 1ObesityPhosphorylationProto-Oncogene Proteins c-aktReceptor, InsulinSignal TransductionThe caveolae-associated coiled-coil protein, NECC2, regulates insulin signalling in Adipocytes.research article30160359open accessAdipocitosAdipogénesisCaveolasAnimalesCaveolina 1Células 3T3-L1FosforilaciónHumanosInsulinaObesidadProteína Quinasa 1 activada por mitógenosProteínas asociadas a microtúbulosReceptor de insulinaResistencia a la insulina10.1111/jcmm.138401582-4934PMC6201366https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jcmm.13840https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201366/pdf