Romeu, Marisa EspinazoCamacho, Antonio LetranAguilar, Carmen MorenoRoger, Aurora JuradoBenitez, Francisco Moreno2023-05-032023-05-032022-05-29Romeu ME, Camacho AL, Aguilar CM, Roger AJ, Benítez FM. Identification of myosin light chain protein as a major fish allergen. Clin Exp Allergy. 2022 Sep;52(9):1101-1104http://hdl.handle.net/10668/19924The prevalence of food allergy has been increasing worldwide in the last 10 years. Currently, 2%–8% of the population in Western countries suffers from some type of food allergy. In Spain, seafood allergy has increased approximately 30% in the last decade, affecting both children and adults, with 10% affected by fish allergy. In this study, we have performed an initial screening of the allergens involved in the sensitization of 21 fish allergic patients from the South of Europe, considering: medical record and positive Skin Prick Test (SPT) to one of these five fish extracts (homemade, online repository): od, hake, tuna, sole and sea bream (Table 1). Western blot was used to recognize the sensitization of these patients and LC/MS was used to characterize the allergen recognized by western blot.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Liquid chromatography-mass spectrometryPrevalenceFood hypersensitivityBlotting, WesternSeafoodMedical recordsAllergensAnimalsFishesMyosin light chainsMyosin-light-chain kinasePhosphorylationresearch article35707958open accessAlérgenosAnimalesCadenas ligeras de miosinaFosforilaciónPecesQuinasa de cadena ligera de miosina10.1111/cea.141861365-2222PMC9545155https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/cea.14186https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545155/pdf