Lebrón, José AntonioLópez-López, ManuelGarcía-Calderón, Clara B.V. Rosado, IvanBalestra, Fernando R.Huertas, PabloRodik, Roman V.Kalchenko, Vitaly I.Bernal, EvaMoyá, María LuisaLópez-Cornejo, PilarOstos, Francisco J.2022-07-262022-07-262021-08-12Lebrón JA, López-López M, García-Calderón CB, V Rosado I, Balestra FR, Huertas P, et al. Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery. Pharmaceutics. 2021 Aug 12;13(8):1250http://hdl.handle.net/10668/3826The formation of calixarene-based liposomes was investigated, and the characterization of these nanostructures was carried out using several techniques. Four amphiphilic calixarenes were used. The length of the hydrophobic chains attached to the lower rim as well as the nature of the polar group present in the upper rim of the calixarenes were varied. The lipid bilayer was formed with one calixarene and with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, DOPE. The cytotoxicity of the liposomes for various cell lines was also studied. From the results obtained, the liposomes formed with the least cytotoxic calixarene, (TEAC12)4, were used as nanocarriers of both nucleic acids and the antineoplastic drug doxorubicin, DOX. Results showed that (TEAC12)4/DOPE/p-EGFP-C1 lipoplexes, of a given composition, can transfect the genetic material, although the transfection efficiency substantially increases in the presence of an additional amount of DOPE as coadjuvant. On the other hand, the (TEAC12)4/DOPE liposomes present a high doxorubicin encapsulation efficiency, and a slow controlled release, which could diminish the side effects of the drug.enAtribución 4.0 InternacionalAtribución 4.0 InternacionalAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Cationic calix[4]arenesLiposomesNucleic acidsTransfection efficiencyDoxorubicinEncapsulationLiposomasÁcidos nucleicosDoxorrubicinaEncapsulación celularMedical Subject Headings::Chemicals and Drugs::Biomedical and Dental Materials::Membranes, Artificial::LiposomesMedical Subject Headings::Chemicals and Drugs::Polycyclic Compounds::Macrocyclic Compounds::CalixarenesMedical Subject Headings::Chemicals and Drugs::Pharmaceutical Preparations::Dosage Forms::Delayed-Action PreparationsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Transfer Techniques::TransfectionMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell LineMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Cyclic::Hydrocarbons, Aromatic::Polycyclic Hydrocarbons, Aromatic::Naphthacenes::Anthracyclines::Daunorubicin::DoxorubicinMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::NanostructuresMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic AgentsMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acidsresearch article34452211open access10.3390/pharmaceutics130812501999-4923PMC8398082