Sabido-Bozo, SusanaPerez-Linero, Ana MariaManzano-Lopez, JavierRodriguez-Gallardo, SofiaAguilera-Romero, AuxiliadoraCortes-Gomez, AlejandroLopez, SergioWellinger, Ralf ErikMuñiz, Manuel2022-09-292022-09-292021-01-03Sabido-Bozo S, Perez-Linero AM, Manzano-Lopez J, Rodriguez-Gallardo S, Aguilera-Romero A, Cortes-Gomez A, et al. The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection. Int J Mol Sci. 2021 Jan 3;22(1):4231661-6596http://hdl.handle.net/10668/4180Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast, Gea1 in the cis-Golgi and Gea2 in the medial-Golgi activate Arf1 to form COPI-coated vesicles for retrograde cargo sorting, whereas Sec7 generates clathrin/adaptor-coated vesicles at the trans-Golgi network (TGN) for forward cargo transport. A central question is how the same activated Arf1 protein manages to assemble different coats depending on the donor Golgi compartment. A previous study has postulated that the interaction between Gea1 and COPI would channel Arf1 activation for COPI vesicle budding. Here, we found that the p24 complex, a major COPI vesicle cargo, promotes the binding of Gea1 with COPI by increasing the COPI association to the membrane independently of Arf1 activation. Furthermore, the p24 complex also facilitates the interaction of Arf1 with its COPI effector. Therefore, our study supports a mechanism by which the p24 complex contributes to program Arf1 activation by Gea1 for selective COPI coat assembly at the cis-Golgi compartment.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/P24 complexCOPIGolgiArfGEFsArf1Proteína coat de complejo IAparato de GolgiFactores de intercambio de guanina nucleótidoFactor 1 de ribosilacion-ADPMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Acid Anhydride Hydrolases::GTP Phosphohydrolases::GTP-Binding Proteins::Monomeric GTP-Binding Proteins::ADP-Ribosylation Factors::ADP-Ribosylation Factor 1Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Cytoplasmic Vesicles::Transport Vesicles::Coated Vesicles::COP-Coated VesiclesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Vesicular Transport Proteins::Coat Protein Complex IMedical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Golgi ApparatusMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::GTP-Binding Protein Regulators::Guanine Nucleotide Exchange FactorsMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Biological Transport::Protein TransportMedical Subject Headings::Organisms::Eukaryota::Fungi::Ascomycota::Saccharomycetales::Saccharomyces::Saccharomyces cerevisiaeMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteinsresearch article33401608Acceso abierto10.3390/ijms220104231422-0067PMC7794930