Lopez-Sanchez, Laura MJimenez-Izquierdo, RafaelPeñarando, JonMena, RafaelGuil-Luna, SilviaToledano, MartaConde, FranciscoVillar, CarlosDiaz, CesarOrtea, IgnacioDe la Haba-Rodriguez, Juan RAranda, EnriqueRodriguez-Ariza, Antonio2023-01-252023-01-252019-09-27López-Sánchez LM, Jiménez-Izquierdo R, Peñarando J, Mena R, Guil-Luna S, Toledano M, et al. SWATH-based proteomics reveals processes associated with immune evasion and metastasis in poor prognosis colorectal tumours. J Cell Mol Med. 2019 Dec;23(12):8219-8232.http://hdl.handle.net/10668/14559Newly emerged proteomic methodologies, particularly data-independent acquisition (DIA) analysis-related approaches, would improve current gene expression-based classifications of colorectal cancer (CRC). Therefore, this study was aimed to identify protein expression signatures using SWATH-MS DIA and targeted data extraction, to aid in the classification of molecular subtypes of CRC and advance in the diagnosis and development of new drugs. For this purpose, 40 human CRC samples and 7 samples of healthy tissue were subjected to proteomic and bioinformatic analysis. The proteomic analysis identified three different molecular CRC subtypes: P1, P2 and P3. Significantly, P3 subtype showed high agreement with the mesenchymal/stem-like subtype defined by gene expression signatures and characterized by poor prognosis and survival. The P3 subtype was characterized by decreased expression of ribosomal proteins, the spliceosome, and histone deacetylase 2, as well as increased expression of osteopontin, SERPINA 1 and SERPINA 3, and proteins involved in wound healing, acute inflammation and complement pathway. This was also confirmed by immunodetection and gene expression analyses. Our results show that these tumours are characterized by altered expression of proteins involved in biological processes associated with immune evasion and metastasis, suggesting new therapeutic options in the treatment of this aggressive type of CRC.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/SWATHcolorectal cancerimmune evasionmesenchymalmetastasisproteomicsAgedBiomarkers, TumorColorectal NeoplasmsFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticGene OntologyGene Regulatory NetworksHumansImmune EvasionKaplan-Meier EstimateMaleMiddle AgedNeoplasm MetastasisPrognosisProteomeProteomicsresearch article31560832open accessAncianoBiomarcadores de tumorEstimación de Kaplan-MeierMetástasis de la neoplasiaNeoplasias colorrectalesOntología de genesPerfilación de la expresión génicaPronósticoProteomaProteómicaRedes reguladoras de genesRegulación neoplásica de la expresión génica10.1111/jcmm.146931582-4934PMC6850959https://doi.org/10.1111/jcmm.14693https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850959/pdf