Gallego-Durán, RocíoÁlvarez-Amor, LeticiaGil-Gómez, AntonioRojas, ÁngelaMuñoz-Hernández, RocíoCádernas-García, AntonioMaya-Miles, DouglasMontero-Vallejo, RocíoGato, SheilaSánchez Torrijos, YolandaAmpuero, JavierMartín, FranciscoRomero-Gómez, Manuel2023-01-252023-01-2520191130-0108http://hdl.handle.net/10668/13743non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.enAlanine TransaminaseAnimalsAspartate AminotransferasesBody WeightCholesterolCholineDiet, High-FatDisease Models, AnimalFructoseLiverMaleMethionineMiceMice, Inbred C57BLNon-alcoholic Fatty Liver DiseaseRandom AllocationSweetening Agentsresearch article30896960open access10.17235/reed.2019.6083/2018https://doi.org/10.17235/reed.2019.6083/2018