Peinado, María ÁngelesOvelleiro, DavidDel Moral, María LuisaHernández, RaquelMartínez-Lara, EstherSiles, EvaPedrajas, José RafaelGarcía-Martín, María LuisaCaro, CarlosPeralta, SebastiánMorales, María EncarnaciónRuiz, María AdolfinaBlanco, Santos2023-05-032023-05-032021-12-27http://hdl.handle.net/10668/21127Exogenous neuroprotective protein neuroglobin (Ngb) cannot cross the blood-brain barrier. To overcome this difficulty, we synthesized hyaluronate nanoparticles (NPs), able to deliver Ngb into the brain in an animal model of stroke (MCAO). These NPs effectively reached neurons, and were microscopically identified after 24 h of reperfusion. Compared to MCAO non-treated animals, those treated with Ngb-NPs showed survival rates up to 50% higher, and better neurological scores. Tissue damage improved with the treatment, but no changes in the infarct volume or in the oxidative/nitrosative values were detected. A proteomics approach (p-valueenAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/MCAOhyaluronate nanoparticlesneuroglobinneuroprotectionproteomicsstrokeAnimalsBlood-Brain BarrierBrain InfarctionEndocytosisGene OntologyInfarction, Middle Cerebral ArteryMagnetic Resonance ImagingMaleNanoparticlesNeuroglobinNeuronsNeuroprotective AgentsNitrosative StressOxidative StressPrincipal Component AnalysisProteomicsRats, WistarStrokeSurvival AnalysisThiobarbituric Acid Reactive Substancesresearch article35008673open access10.3390/ijms230102471422-0067PMC8745106https://www.mdpi.com/1422-0067/23/1/247/pdf?version=1641454198https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745106/pdf