Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice.

Rodriguez, Maria JWangorsch, AndreaGomez, FranciscaSchulke, StefanTorres, Maria JVieths, StefanScheurer, StephanToda, MasakoMayorga, Cristobalina2023-01-252023-01-252018-06-13Rodriguez MJ, Wangorsch A, Gomez F, Schülke S, Torres MJ, Vieths S, et al. Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice. J Immunol Res. 2018 Jun 13;2018:3479185http://hdl.handle.net/10668/12711The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Prunus persicaT-Lymphocytes, Helper-InducerT-Lymphocytes, RegulatoryTh2 CellsAnaphylaxisAnimalsAntigens, PlantDesensitization, ImmunologicDisease Models, AnimalFemaleFood HypersensitivityImmunoglobulin EImmunoglobulin GMiceMice, Inbred BALB CPlant ProteinsImmunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice.research article30009186open accessInmunoglobulina GHipersensibilidadInmunoterapiaInmunoglobulina EAnafilaxia10.1155/2018/34791852314-7156PMC6020533https://doi.org/10.1155/2018/3479185https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020533/pdf