Thirion, LaurencePezzi, LauraPedrosa-Corral, IreneSanbonmatsu-Gamez, Sarade Lamballerie, XavierFalchi, AlessandraPerez-Ruiz, MercedesCharrel, Remi N.2022-07-042022-07-042021-02-24Thirion L, Pezzi L, Pedrosa-Corral I, Sanbonmatsu-Gamez S, de Lamballerie X, Falchi A, et al. Evaluation of a Trio Toscana Virus Real-Time RT-PCR Assay Targeting Three Genomic Regions within Nucleoprotein Gene. Pathogens. 2021 Feb 24;10(3):254http://hdl.handle.net/10668/3732Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene. Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes. In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples. In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/PhlebovirusPhenuiviridaeMeningitisSand flyPhlebotomusArbovirusMediterraneanDiagnosticsPsychodidaeDiagnósticoMedical Subject Headings::Organisms::Viruses::RNA Viruses::Bunyaviridae::Phlebovirus::Sandfly fever Naples virusMedical Subject Headings::Diseases::Bacterial Infections and Mycoses::Central Nervous System InfectionsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::NucleoproteinsMedical Subject Headings::Information Science::Information Science::Medical Informatics::Medical Informatics Applications::Information Storage and Retrieval::Data MiningMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNAresearch article33668339Acceso abierto10.3390/pathogens100302542076-0817PMC7996202