Pleguezuelos-Beltran, PaulaGalvez-Martin, PatriciaNieto-Garcia, DanielMarchal, Juan AntonioLopez-Ruiz, Elena2023-05-032023-05-032022-02-18Pleguezuelos-Beltrán P, Gálvez-Martín P, Nieto-García D, Marchal JA, López-Ruiz E. Advances in spray products for skin regeneration. Bioact Mater. 2022 Mar 8;16:187-203.http://hdl.handle.net/10668/22032To date, skin wounds are still an issue for healthcare professionals. Although numerous approaches have been developed over the years for skin regeneration, recent advances in regenerative medicine offer very promising strategies for the fabrication of artificial skin substitutes, including 3D bioprinting, electrospinning or spraying, among others. In particular, skin sprays are an innovative technique still under clinical evaluation that show great potential for the delivery of cells and hydrogels to treat acute and chronic wounds. Skin sprays present significant advantages compared to conventional treatments for wound healing, such as the facility of application, the possibility to treat large wound areas, or the homogeneous distribution of the sprayed material. In this article, we review the latest advances in this technology, giving a detailed description of investigational and currently commercially available acellular and cellular skin spray products, used for a variety of diseases and applying different experimental materials. Moreover, as skin sprays products are subjected to different classifications, we also explain the regulatory pathways for their commercialization and include the main clinical trials for different skin diseases and their treatment conditions. Finally, we argue and suggest possible future trends for the biotechnology of skin sprays for a better use in clinical dermatology.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/ATMP, advanced therapy medicinal productBLA, Biologic License ApplicationCAT, Committee for Advances TherapiesCEA, cultured epithelial autograftCFR, Code of Federal RegulationsCHMP, Committee for Medicinal Product for Human UseCTD, Common Technical DocumentDMEM, Dulbecco's Modified Eagle's mediumECM, extracellular matrixEMA, European Medicines AgencyEU, European UnionFDA, Food and Drug AdministrationFibrinGAGs, glycosaminoglycansGLP, Good Laboratory PracticeGMP, Good Manufacturing PracticeHA, hyaluronic acidHCT/Ps, human cells, tissues, and cellular and tissue-based productsHydrogelISO, International Organization for StandardizationMA, marketing authorizationNP, Notified bodyOTAT, Office of Tissues and Advanced TherapiesPCL, polycaprolactonePEG, polyethylene glycolPHSA, Public Health Service ActPMA, Premarket ApprovalPRF, platelet rich fibrinPRP, platelet rich plasmaPU, polyurethaneQMS, Quality Management SystemSkinSprayTE, tissue engineeringTissue engineeringUSA, United States of AmericaHumansRegenerative MedicineSkin, ArtificialHydrogelsBioprintingDermatologyWound HealingSkin DiseasesSkin AbnormalitiesBiotechnologyDelivery of Health CareAdvances in spray products for skin regeneration.research article35386328open accessAtención a la saludAnomalías cutáneasBioimpresiónBiotecnologíaCicatrización de heridasDermatologíaEnfermedades de la pielHidrogelesHumanosMedicina regenerativaPiel artificial10.1016/j.bioactmat.2022.02.0232452-199XPMC8965724https://digibug.ugr.es/bitstream/10481/74910/1/1-s2.0-S2452199X22000974-main.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965724/pdf