Sharifi, SinaIslam, Mohammad MirazulSharifi, HannahIslam, RakibulKoza, DarrellReyes-Ortega, FelisaAlba-Molina, DavidNilsson, Per H.Dohlman, Claes H.Mollnes, Tom EirikChodosh, JamesGonzalez-Andrades, Miguel2023-01-112023-01-112021-04-17Sharifi S, Islam MM, Sharifi H, Islam R, Koza D, Reyes-Ortega F, et al. Tuning gelatin-based hydrogel towards bioadhesive ocular tissue engineering applications. Bioact Mater. 2021 Apr 17;6(11):3947-39612097-1192http://hdl.handle.net/10668/4569Gelatin based adhesives have been used in the last decades in different biomedical applications due to the excellent biocompatibility, easy processability, transparency, non-toxicity, and reasonable mechanical properties to mimic the extracellular matrix (ECM). Gelatin adhesives can be easily tuned to gain different viscoelastic and mechanical properties that facilitate its ocular application. We herein grafted glycidyl methacrylate on the gelatin backbone with a simple chemical modification of the precursor, utilizing epoxide ring-opening reactions and visible light-crosslinking. This chemical modification allows the obtaining of an elastic protein-based hydrogel (GELGYM) with excellent biomimetic properties, approaching those of the native tissue. GELGYM can be modulated to be stretched up to 4 times its initial length and withstand high tensile stresses up to 1.95 MPa with compressive strains as high as 80% compared to Gelatin-methacryloyl (GeIMA), the most studied derivative of gelatin used as a bioadhesive. GELGYM is also highly biocompatible and supports cellular adhesion, proliferation, and migration in both 2 and 3-dimensional cell-cultures. These characteristics along with its super adhesion to biological tissues such as cornea, aorta, heart, muscle, kidney, liver, and spleen suggest widespread applications of this hydrogel in many biomedical areas such as transplantation, tissue adhesive, wound dressing, bioprinting, and drug and cell delivery.enAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Natural-based hydrogelGelatinBiocompatibleBiomimeticBioadhesiveCorneaTissue adhesivesMethacrylatesLightHidrogelesGelatinaMateriales biocompatiblesBiomiméticaCórneaAdhesivos tisularesMetacrilatosLuzMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Scleroproteins::GelatinMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::BioprintingMedical Subject Headings::Chemicals and Drugs::Complex Mixtures::Colloids::Gels::HydrogelsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Surgical Equipment::Surgical Fixation Devices::Tissue AdhesivesMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::AdhesivesMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biotechnology::BiomimeticsMedical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::SpleenMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Acids, Acyclic::Acrylates::MethacrylatesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::BandagesMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Ethers::Ethers, Cyclic::Epoxy CompoundsMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Optical Phenomena::LightMedical Subject Headings::Anatomy::Digestive System::LiverMedical Subject Headings::Anatomy::Sense Organs::Eye::Anterior Eye Segment::CorneaMedical Subject Headings::Anatomy::Cardiovascular System::Blood Vessels::Arteries::AortaMedical Subject Headings::Anatomy::Urogenital System::Urinary Tract::Kidneyresearch article33937594open access10.1016/j.bioactmat.2021.03.0422452-199XPMC8080056