Peran, LauraSierra, SaletaComalada, MònicaLara-Villoslada, FedericoBailón, ElviraNieto, AnaConcha, AngelOlivares, MónicaZarzuelo, AntonioXaus, JordiGálvez, Julio2012-11-222012-11-222007-01-12Peran L, Sierra S, Comalada M, Lara-Villoslada F, Bailón E, Nieto A, et al. A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis. Br. J. Nutr.. 2007 ; 97(1):96-1030007-1145http://hdl.handle.net/10668/646Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;The intestinal anti-inflammatory effects of two probiotics isolated from breast milk, Lactobacillus reuteri and L. fermentum, were evaluated and compared in the trinitrobenzenesulfonic acid (TNBS) model of rat colitis. Colitis was induced in rats by intracolonic administration of 10 mg TNBS dissolved in 50% ethanol (0.25 ml). Either L. reuteri or L. fermentum was daily administered orally (5 x 10(8) colony-forming units suspended in 0.5 ml skimmed milk) to each group of rats (n 10) for 3 weeks, starting 2 weeks before colitis induction. Colonic damage was evaluated histologically and biochemically, and the colonic luminal contents were used for bacterial studies and for SCFA production. Both probiotics showed intestinal anti-inflammatory effects in this model of experimental colitis, as evidenced histologically and by a significant reduction of colonic myeloperoxidase activity (P<0.05). L. fermentum significantly counteracted the colonic glutathione depletion induced by the inflammatory process. In addition, both probiotics lowered colonic TNFalpha levels (P<0.01) and inducible NO synthase expression when compared with non-treated rats; however, the decrease in colonic cyclo-oxygenase-2 expression was only achieved with L.fermentum administration. Finally, the two probiotics induced the growth of Lactobacilli species in comparison with control colitic rats, but the production of SCFA in colonic contents was only increased when L. fermentum was given. In conclusion, L. fermentum can exert beneficial immunomodulatory properties in inflammatory bowel disease, being more effective than L. reuteri, a probiotic with reputed efficacy in promoting beneficial effects on human health.enProbioticsInflammatory bowel diseasesImmunomodulationAnti-inflammatory activityColitisColonDiarreaÁcidos Grasos VolátilesHecesFemeninoContenido DigestivoGlutatiónConcentración de Iones de HidrógenoEnfermedades Inflamatorias del IntestinoMucosa IntestinalLactobacillus fermentumLactobacillus reuteriModelos AnimalesMúsculo EsqueléticoTamaño de los ÓrganosPeroxidasaProbióticosRatasRatas WistarBazoTimoÁcido TrinitrobencenosulfónicoFactor de Necrosis Tumoral alfaAnimalesMedical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Gastroenteritis::ColitisMedical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestine, Large::ColonMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Digestive::DiarrheaMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, VolatileMedical Subject Headings::Anatomy::Fluids and Secretions::FecesMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Anatomy::Fluids and Secretions::Gastrointestinal ContentsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Oligopeptides::GlutathioneMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Hydrogen-Ion ConcentrationMedical Subject Headings::Diseases::Digestive System Diseases::Gastrointestinal Diseases::Intestinal Diseases::Inflammatory Bowel DiseasesMedical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestinal MucosaMedical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus fermentumMedical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus::Lactobacillus reuteriMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, AnimalMedical Subject Headings::Anatomy::Musculoskeletal System::Muscles::Muscle, SkeletalMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ SizeMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::PeroxidaseMedical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Supplements::ProbioticsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::RatsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::RatsMedical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::SpleenMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Nitro Compounds::Nitrobenzenes::Trinitrobenzenes::Trinitrobenzenesulfonic AcidMedical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Tumor Necrosis Factors::Tumor Necrosis Factor-alphaMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Glandresearch article17217564open access10.1017/S00071145072577701475-2662