Cárdenas, CasimiroTorres-Vargas, José AntonioCárdenas-Valdivia, AbelJurado, NuriaQuesada, Ana R.García-Caballero, MelissaMartínez-Poveda, BeatrizMedina, Miguel Ángel2023-01-182023-01-182021-10-06Cárdenas C, Torres-Vargas JA, Cárdenas-Valdivia A, Jurado N, Quesada AR, García-Caballero M, et al. Non-targeted metabolomics characterization of Annona muricata leaf extracts with anti-angiogenic activity. Biomed Pharmacother. 2021 Dec;144:1122630753-3322http://hdl.handle.net/10668/4594The tropical plant Annona muricata has been widely used for traditional ethnobotanic and pharmacologic applications. Extracts from different parts of this plant have been shown to have a wide range of biological activities. In the present study, we carry out a metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves that has allowed us to identify 33 bioactive compounds. Furthermore, we have shown that aqueous extracts are able to inhibit endothelial cell migration and both aqueous and DMSO extracts inhibit the formation of tubule-like structures by endothelial cells cultured on Matrigel. We conclude that extracts of Annona muricata leaves have great potential as anti-angiogenic natural combinations of bioactive compounds.enAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Ultra-high-performance liquidChromatography-high-resolution mass spectrometry (UHPLC–HRMS)Annona muricataGraviolaSoursopAngiogenesisCromatografía líquida de alta presiónEspectrometría de masasAnnonaceaeAnnonaNeovascularización fisiológicaMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Growth Substances::Angiogenesis Modulating Agents::Angiogenesis InhibitorsMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Artiodactyla::Ruminants::CattleMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell DifferentiationMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell MovementMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Chromatography::Chromatography, Liquid::Chromatography, High Pressure LiquidMedical Subject Headings::Anatomy::Cells::Epithelial Cells::Endothelial CellsMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::MetabolomeMedical Subject Headings::Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Cardiovascular Physiological Phenomena::Cardiovascular Physiological Processes::Neovascularization, PhysiologicMedical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::PhytochemicalsMedical Subject Headings::Chemicals and Drugs::Complex Mixtures::Biological Products::Plant Preparations::Plant ExtractsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Spectrometry, Mass, Electrospray IonizationMedical Subject Headings::Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Annonaceae::Annonaresearch article34626933Acceso abierto10.1016/j.biopha.2021.1122631950-6007