Perez, CristinaBotta, CirinoZabaleta, AintzanePuig, NoemiCedena, Maria-TeresaGoicoechea, IbaiAlameda, DanielSan José-Eneriz, EdurneMerino, JuanaRodríguez-Otero, PaulaMaia, CatarinaAlignani, DiegoMaiso, PatriciaManrique, IreneLara-Astiaso, DavidVilas-Zornoza, AmaiaSarvide, SaraiRiillo, CaterinaRossi, MarcoRosiñol, LauraOriol, AlbertBlanchard, María-JesúsRios, RafaelSureda, AnnaMartin, JesusMartinez, RafaelBargay, Joande la Rubia, JavierHernandez, Miguel-TeodoroMartinez-Lopez, JoaquinOrfao, AlbertoAgirre, XabierProsper, FelipeMateos, Maria-VictoriaLahuerta, Juan-JoséBlade, JoanSan-Miguel, Jesús FPaiva, Bruno2023-02-082023-02-082020http://hdl.handle.net/10668/15418Granulocytic myeloid-derived suppressor cells (G-MDSCs) promote tumor growth and immunosuppression in multiple myeloma (MM). However, their phenotype is not well established for accurate monitoring or clinical translation. We aimed to provide the phenotypic profile of G-MDSCs based on their prognostic significance in MM, immunosuppressive potential, and molecular program. The preestablished phenotype of G-MDSCs was evaluated in bone marrow samples from controls and MM patients using multidimensional flow cytometry; surprisingly, we found that CD11b+CD14-CD15+CD33+HLADR- cells overlapped with common eosinophils and neutrophils, which were not expanded in MM patients. Therefore, we relied on automated clustering to unbiasedly identify all granulocytic subsets in the tumor microenvironment: basophils, eosinophils, and immature, intermediate, and mature neutrophils. In a series of 267 newly diagnosed MM patients (GEM2012MENOS65 trial), only the frequency of mature neutrophils at diagnosis was significantly associated with patient outcome, and a high mature neutrophil/T-cell ratio resulted in inferior progression-free survival (PenAntigens, CDFemaleFollow-Up StudiesHumansLymphocyte CountMaleMiddle AgedMultiple MyelomaMyeloid-Derived Suppressor CellsNeoplasm ProteinsNeutrophilsT-LymphocytesTranscription, Geneticresearch article32325491open access10.1182/blood.20190045371528-0020https://ashpublications.org/blood/article-pdf/136/2/199/1747998/bloodbld2019004537.pdf