Mascaraque, AinhoaKowalczyk, WioletaFernández, TahiaPalomares, FranciscaMayorga, CristobalinaAndreu, DavidRojo, Javier2016-07-132016-07-132015-10Masqueraque A, Kowalczyk V, Fernández T. Palomares F,Mayorga C, Andreu D. Glycodendropeptides stimulate dendritic cell maturtion and T cell proliferation: a potential influenza A virus immunotherapy. MedChemComm. 2015; 6. 1755-1760.2040-2511http://hdl.handle.net/10668/2274Mannosylation facilitates uptake and internalization of immunogenic peptides by antigen-processing cells expressing mannose receptors at their surface, such as DC-SIGN, a lectin that plays a key role in the immune response against different pathogens. This internalization, processing and subsequent MHC presentation may result in a strong T cell stimulation. Here, we hypothesized that combining mannose glycodendrons with multivalent presentation of peptide epitopes in a likewise dendron format would yield hybrid constructs, named glycodendropeptides (GDPs), with the capacity to enhance peptide immunogenicity, hence providing a novel and versatile platform for applications in immunotherapy. Thus, GDPs of different valencies displaying the NP366–374 epitope, a conserved sequence from the influenza A virus nucleoprotein (NP), have been built by two click chemistry-based methodologies and assessed as potential flu vaccine candidates. Preliminary evaluation of the ability of these constructs to stimulate dendritic cell maturation and lymphocyte proliferation was promising, showing the highest-functionalized NP366–374 GDPs as inducing the strongest immunostimulatory effect.enMoléculas de adhesión celularQuímica clicSecuencia conservadaDendrímerosCélulas dendríticasEpítoposInmunoterapiaLectinas tipo CManosaLectinas de unión a manosaPéptidosProteínas de unión al ARNReceptores de superficie celularLinfocitos TVacunasProteínas del centro víricoMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Membrane Glycoproteins::Cell Adhesion MoleculesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Technology, Pharmaceutical::Click ChemistryMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Conserved SequenceMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Drug Therapy::Drug Delivery Systems::Drug Carriers::DendrimersMedical Subject Headings::Anatomy::Cells::Epithelial Cells::Dendritic CellsMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::EpitopesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Immunomodulation::ImmunotherapyMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Lectins::Lectins, C-TypeMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Monosaccharides::Hexoses::MannoseMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Lectins::Mannose-Binding LectinsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::PeptidesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nucleoproteins::RNA-Binding ProteinsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell SurfaceMedical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-LymphocytesMedical Subject Headings::Chemicals and Drugs::Complex Mixtures::Biological Agents::VaccinesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Viral Proteins::Viral Structural Proteins::Nucleocapsid Proteins::Viral Core Proteinsresearch articleopen access10.1039/C5MD00133A2040-2503