Mayas Torres, María DoloresQueipo-Ortuño, María IsabelClemente-Postigo, MercedesMacías González, ManuelEl Bekay, RajaaTinahones, Francisco JCardona Díaz, Fernando2013-03-182013-03-182011-03Mayas MD, Queipo-Ortuño MI, Clemente-Postigo M, Macias M, El Bekay R, Tinahones FJ, Cardona F. Influence of a fat overload on lipogenic regulators in metabolic syndrome patients. Br. J. Nutr.. 2011; 105(6):895-9010007-1145http://hdl.handle.net/10668/846Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't;Several epidemiological studies have related an increase of lipids in the postprandial state to an individual risk for the development of CVD, possibly due to the increased plasma levels of TAG and fatty acids (FA) through enzymes of FA metabolism. The interaction between nutrition and the human genome determines gene expression and metabolic response. The aim of the present study was to evaluate the influence of a fat overload on the gene mRNA levels of lipogenic regulators in peripheral blood mononuclear cells (PBMC) from patients with the metabolic syndrome. The study included twenty-one patients with criteria for the metabolic syndrome who underwent a fat overload. Measurements were made before and after the fat overload of anthropometric and biochemical variables and also the gene mRNA levels of lipogenic factors. The main results were that the fat overload led to an increased mRNA levels of sterol regulatory element binding protein-1 (SREBP1), retinoid X receptor α (RXRα) and liver X receptor α (LXRα) in PBMC, and this increase was associated with the FA synthase (FASN) mRNA levels. We also found that TAG levels correlated with FASN mRNA levels. In addition, there was a positive correlation of SREBP1 with RXRα and of LXRα with the plasma lipoperoxide concentration. The fat overload led to an increase in regulators of lipogenesis in PBMC from patients with the metabolic syndrome.enComplejo Ácido Graso SintetasaFemeninoHumanosLeucocitos MononuclearesPeróxidos LipídicosLipogénesisMasculinoSíndrome X MetabólicoReceptores Nucleares HuérfanosARN MensajeroReceptores X RetinoideProteínas de Unión a los Elementos Reguladores de EsterolesTriglicéridosGrasas en la DietaGenetic expressionPostprandial hypertriacylglycerolaemiaLipogenic genesMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Multiprotein Complexes::Multienzyme Complexes::Fatty Acid Synthetase ComplexMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, MononuclearMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Free Radicals::Peroxides::Lipid PeroxidesMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::LipogenesisMedical Subject Headings::Check Tags::MaleMedical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Metabolic Syndrome XMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Orphan Nuclear ReceptorsMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, MessengerMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Receptors, Retinoic Acid::Retinoid X ReceptorsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic Helix-Loop-Helix Transcription Factors::Basic Helix-Loop-Helix Leucine Zipper Transcription Factors::Sterol Regulatory Element Binding ProteinsMedical Subject Headings::Chemicals and Drugs::Lipids::Glycerides::TriglyceridesMedical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Fatsresearch article21114893open access10.1017/S00071145100045141475-2662