Cabeza, LauraPeña, MercedesMartínez, RosarioMesas, CristinaGalisteo, MilagrosPerazzoli, GloriaPrados, JosePorres, Jesús M.Melguizo, Consolación2022-09-212022-09-212021-02-08Cabeza L, Peña M, Martínez R, Mesas C, Galisteo M, Perazzoli G, et al. Anemonia sulcata and Its Symbiont Symbiodinium as a Source of Anti-Tumor and Anti-Oxoxidant Compounds for Colon Cancer Therapy: A Preliminary in Vitro Study. Biology. 2021 Feb 8;10(2):134.http://hdl.handle.net/10668/4088Recently, invertebrate marine species have been investigated for the presence of natural products with antitumor activity. We analyzed the invertebrate Anemonia sulcata with (W) and without (W/O) the presence of its microalgal symbiont Symbiodinium as a source of bioactive compounds that may be applied in the therapy and/or prevention of colorectal cancer (CRC). Animals were mechanically homogenized and subjected to ethanolic extraction. The proximate composition and fatty acid profile were determined. In addition, an in vitro digestion was performed to study the potentially dialyzable fraction. The antioxidant and antitumor activity of the samples and the digestion products were analyzed in CRC cells in vitro. Our results show a high concentration of polyunsaturated fatty acid in the anemone and a great antioxidant capacity, which demonstrated the ability to prevent cell death and a high antitumor activity of the crude homogenates against CRC cells and multicellular tumor spheroids, especially W/O symbiont. These preliminary results support that Anemonia sulcata could be a source of bioactive compounds with antioxidant and antitumor potential against CRC and that the absence of its symbiont may enhance these properties. Further studies will be necessary to define the bioactive compounds of Anemonia sulcata and their mechanisms of action.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Anemonia sulcataSymbiodiniumAntioxidant activityAntitumor activityColorectal cancerCell deathDigestionAnemoneAntioxidantesNeoplasias colorrectalesMuerte celularDigestiónMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Cnidaria::Anthozoa::Sea AnemonesMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::AntioxidantsMedical Subject Headings::Chemicals and Drugs::Complex Mixtures::Biological ProductsMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty AcidsMedical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, UnsaturatedMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Gastrointestinal Neoplasms::Intestinal Neoplasms::Colorectal NeoplasmsMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell DeathMedical Subject Headings::Phenomena and Processes::Digestive System and Oral Physiological Phenomena::Digestive System Physiological Phenomena::Digestive System Processes::DigestionMedical Subject Headings::Publication Type::Study Characteristics::In Vitroresearch article33567702Acceso abierto10.3390/biology100201342079-7737PMC7915377