Mora-Castilla, SergioTejedo, Juan RTapia-Limonchi, RafaelDíaz, IreneHitos, Ana BCahuana, Gladys MHmadcha, AbdelkrimMartín, FranzSoria, BernatBedoya, Francisco J2016-07-052016-07-052014-12-03Mora-Castilla S, Tejedo JR, Tapia-Limonchi R, Díaz I, Hitos AB, Cahuana GM, et al. Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation. Stem Cells Int; 2014:379678http://hdl.handle.net/10668/2253Journal Article;The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols. Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression. Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition.enCélulas madre embrionariasLínea celularEndodermoProteínas FetalesHistonasMedical Subject Headings::Anatomy::Cells::Stem Cells::Pluripotent Stem Cells::Embryonic Stem Cells::Mouse Embryonic Stem CellsMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell LineMedical Subject Headings::Anatomy::Embryonic Structures::Germ Layers::EndodermMedical Subject Headings::Anatomy::Embryonic Structures::Germ Layers::MesodermMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Fetal ProteinsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nuclear Proteins::HistonesMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, MessengerMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::T-Box Domain Proteinsresearch article25544848open access10.1155/2014/3796781687-966XPMC4269087