Toribio, María JoséPriego-Capote, FelicianoPérez-Gómez, BeatrizFernández de Larrea-Baz, NereaRuiz-Moreno, EmmaCastelló, AdelaLucas, PilarSierra, María ÁngelesPino, Marina NievesMartínez-Cortés, MercedesLuque de Castro, María DoloresLope, VirginiaPollán, Marina2022-09-222022-09-222021-10-23Toribio MJ, Priego-Capote F, Pérez-Gómez B, Fernández de Larrea-Baz N, Ruiz-Moreno E, Castelló A, et al. Factors Associated with Serum Vitamin D Metabolites and Vitamin D Metabolite Ratios in Premenopausal Women. Nutrients. 2021 Oct 23;13(11):3747http://hdl.handle.net/10668/4111The most representative indicator of vitamin D status in clinical practice is 25(OH)D3, but new biomarkers could improve the assessment of vitamin D status and metabolism. The objective of this study is to investigate the association of serum vitamin D metabolites and vitamin D metabolite ratios (VMRs) with potentially influential factors in premenopausal women. This is a cross-sectional study based on 1422 women, aged 39-50, recruited from a Madrid Medical Diagnostic Center. Participants answered an epidemiological and a food frequency questionnaire. Serum vitamin D metabolites were determined using an SPE-LC-MS/MS platform. The association between participant's characteristics, vitamin D metabolites, and VMRs was quantified by multiple linear regression models. Mean 25(OH)D3 concentration was 49.2 + 18.9 nmol/L, with greater deficits among obese, nulliparous, dark-skinned women, and with less sun exposure. A lower R2 ratio (1,25(OH)2D3/25(OH)D3) and a higher R4 (24,25(OH)2D3/1,25(OH)2D3) were observed in nulliparous women, with high sun exposure, and those with low caloric intake or high consumption of calcium, vitamin D supplements, or alcohol. Nulliparous women had lower R1 (25(OH)D3/Vit D3) and R3 (24,25(OH)2D3/25(OH)D3), and older women showed lower R3 and R4. Vitamin D status modified the association of the VMRs with seasons. VMRs can be complementary indicators of vitamin D status and its endogenous metabolism, and reveal the influence of certain individual characteristics on the expression of hydroxylase enzymes.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Vit D325(OH)D31,25(OH)2D324,25(OH)2D3Vitamin D metabolite ratiosColecalciferolCalcifediolCalcitriol24,25-Dihidroxivitamina D 3Vitamina DMedical Subject Headings::Persons::Persons::Age Groups::AdultMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Persons::Persons::Age Groups::Adult::Middle AgedMedical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Sexual Development::Climacteric::Menopause::PremenopauseMedical Subject Headings::Chemicals and Drugs::Polycyclic Compounds::Steroids::Cholestanes::Cholestenes::Cholecalciferol::Hydroxycholecalciferols::Dihydroxycholecalciferols::24,25-Dihydroxyvitamin D 3Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolomeresearch article34836003open access10.3390/nu131137472072-6643PMC8621214