Vega, AntonioChacón, PedroMonteseirín, JavierEl Bekay, RajaaAlba, GonzaloMartín-Nieto, JoséSobrino, Francisco2013-10-232013-10-232007-07-15Vega A, Chacón P, Monteseirín J, El Bekay R, Alba G, Martín-Nieto J, et al. Expression of the transcription factor NFAT2 in human neutrophils: IgE-dependent, Ca2+- and calcineurin-mediated NFAT2 activation. J. Cell. Sci.. 2007; 120(Pt 14):2328-370021-9533http://hdl.handle.net/10668/1343Journal Article; Research Support, Non-U.S. Gov't;NFAT (nuclear factors of activated T cells) proteins constitute a family of transcription factors involved in mediating signal transduction. The presence of NFAT isoforms has been described in all cell types of the immune system, with the exception of neutrophils. In the present work we report for the first time the expression in human neutrophils of NFAT2 mRNA and protein. We also report that specific antigens were able to promote NFAT2 protein translocation to the nucleus, an effect that was mimicked by the treatment of neutrophils with anti-immunoglobulin E (anti-IgE) or anti-Fcepsilon-receptor antibodies. Antigens, anti-IgE and anti-FcepsilonRs also increased Ca2+ release and the intracellular activity of calcineurin, which was able to interact physically with NFAT2, in parallel to eliciting an enhanced NFAT2 DNA-binding activity. In addition, specific chemical inhibitors of the NFAT pathway, such as cyclosporin A and VIVIT peptide, abolished antigen and anti-IgE-induced cyclooxygenase-2 (COX2) gene upregulation and prostaglandin (PGE(2)) release, suggesting that this process is through NFAT. Our results provide evidence that NFAT2 is constitutively expressed in human neutrophils, and after IgE-dependent activation operates as a transcription factor in the modulation of genes, such as COX2, during allergic inflammation.enNeutrophilsNFATCalcineurinAllergyCiclooxigenasa 2Activación NeutrófilaTranscripción GenéticaInmunoglobulina ENúcleo celularRegulación de la expresión génicaCalcioFactores de transcripción NFATCMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::CalcineurinMedical Subject Headings::Chemicals and Drugs::Biological Factors::Blood Coagulation Factors::CalciumMedical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cell NucleusMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Multienzyme Complexes::Prostaglandin-Endoperoxide Synthases::Cyclooxygenase 2Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression RegulationMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin EMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::NFATC Transcription FactorsMedical Subject Headings::Phenomena and Processes::Immune System Phenomena::Immune System Processes::Neutrophil ActivationMedical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::NeutrophilsMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression::Transcription, GeneticMedical Subject Headings::Named Groups::Persons::Age Groups::Adultresearch article17606988open access10.1242/jcs.0003311477-9137