Lebrón, José AntonioOstos, Francisco JoséLópez-López, ManuelMoyá, María LuisaSales, CarlosGarcía, EncarnaciónGarcía-Calderón, Clara BeatrizGarcía-Calderón, MargaritaPeña-Gómez, María JoséRosado, Iván V.Balestra, Fernando R.Huertas, PabloLópez-Cornejo, Pilar2022-11-212022-11-212020-05-25Lebrón JA, Ostos FJ, López-López M, Moyá ML, Sales C, García E, et al. Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material. Pharmaceutics. 2020 May 25;12(5):482http://hdl.handle.net/10668/4380Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+ complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between Kapp and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide's condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Non-toxic nanocarriersSpecificity by cancer cellsGene therapyTransfection processMetallo-liposomesRuthenium(II)-based lipidsPolynucleotidesLiposomesDNATerapia genéticaPolinucleótidosLiposomasADNRutenioTransfecciónVectores genéticosMedical Subject Headings::Chemicals and Drugs::Biomedical and Dental Materials::Membranes, Artificial::LiposomesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Transfer Techniques::TransfectionMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNAMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::PlasmidsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Electrochemical Techniques::Electrophoresis::Electrophoresis, Agar GelMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell LineMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Genetic TherapyMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleotides::PolynucleotidesMedical Subject Headings::Chemicals and Drugs::Lipids::PhospholipidsMedical Subject Headings::Diseases::NeoplasmsMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Ruthenium CompoundsMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genetic Vectorsresearch article32466339open access10.3390/pharmaceutics120504821999-4923PMC7284339