Padial-Molina, MiguelCrespo-Lora, VicenteCandido-Corral, ClaraMartin-Morales, NatiAbril-Garcia, DarioGalindo-Moreno, PabloHernandez-Cortes, PedroO'Valle, Francisco2022-11-222022-11-222021-03-26Padial-Molina M, Crespo-Lora V, Candido-Corral C, Martin-Morales N, Abril-Garcia D, Galindo-Moreno P, et al. Expression of Musashi-1 Increases in Bone Healing. Int J Mol Sci. 2021 Mar 26;22(7):33951661-6596http://hdl.handle.net/10668/4388Musashi-1 (MSI1) is an RNA-binding protein that regulates progenitor cells in adult and developing organisms to maintain self-renewal capacities. The role of musashi-1 in the bone healing environment and its relation with other osteogenic factors is unknown. In the current study, we analyze the expression of MSI1 in an experimental model of rat femoral bone fractures. We also analyze the relation between MSI1 expression and the expression of two osteogenic markers: periostin (POSTN) and runt-related transcription factor 2 (RUNX2). We use histological, immunohistochemical, and qPCR techniques to evaluate bone healing and the expression of MSI1, POSTN, and RUNX2 over time (4, 7, and 14 days). We compare our findings with non-fractured controls. We find that in bone calluses, the number of cells expressing MSI1 and RUNX2 increase over time and the intensity of POSTN expression decreases over time. Within bone calluses, we find the presence of MSI1 expression in mesenchymal stromal cells, osteoblasts, and osteocytes but not in hypertrophic chondrocytes. After 14 days, the expression of MSI1, POSTN, and RUNX2 was significantly correlated. Thus, we conclude that musashi-1 potentially serves in the osteogenic differentiation of mesenchymal stromal cells and bone healing. Therefore, further studies are needed to determine the possibility of musashi-1's role as a clinical biomarker of bone healing and therapeutic agent for bone regeneration.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Musashi-1Runx2PeriostinMesenchymal stem cellsBone healingProteínas de unión al ARNSubunidad alfa 2 del factor de unión al sitio principalProteínaCélulas madre mesenquimatosasCuración de fracturaMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Bone and Bones::Bony CallusMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Cell Adhesion MoleculesMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::ChondrocytesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::Core Binding Factors::Core Binding Factor alpha Subunits::Core Binding Factor Alpha 1 SubunitMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nerve Tissue ProteinsMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::OsteoblastsMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Osteoblasts::OsteocytesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::RNA-Binding ProteinsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats, WistarMedical Subject Headings::Phenomena and Processes::Biological Phenomena::Biological Processes::Regeneration::Wound Healing::Fracture HealingMedical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Bone Development::Osteogenesisresearch article33810326open access10.3390/ijms220733951422-0067PMC8037090