Ortiz-Arrabal, OlimpiaChato-Astrain, JesusCrespo, Pascual VicenteGarzon, IngridMesa-Garcia, Maria DoloresAlaminos, MiguelGomez-Llorente, Carolina2023-05-032023-05-032022-04-08Ortiz-Arrabal O, Chato-Astrain J, Crespo PV, Garzón I, Mesa-García MD, Alaminos M, et al. Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering. Front Bioeng Biotechnol. 2022 Apr 27;10:876734.2296-4185http://hdl.handle.net/10668/20523In the present work, we evaluated the potential of maslinic acid (MA) to improve currently available keratinocyte culture methods for use in skin tissue engineering. Results showed that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24, 48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This effect was associated to a significant increase of KI-67 protein expression and upregulation of several genes associated to cell proliferation (PCNA) and differentiation (cytokeratins, intercellular junctions and basement membrane related genes). When human keratinocytes were isolated from skin biopsies, we found that MA at the concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell dissociation methods. These results revealed the positive effects of MA to optimize human keratinocyte culture protocols for use in skin tissue engineering.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/cell culturecell proliferationepithelial cellsmaslinic acidtissue engineeringHumansHumansKi-67 AntigenProliferating Cell Nuclear AntigenTissue EngineeringBasement MembraneCell SurvivalUp-Regulationmaslinic acidKeratinocytesKeratinsCell Proliferationresearch article35662841open accessAntígeno Ki-67Antígeno nuclear de célula en proliferaciónHumanosIngeniería de tejidosMembrana basalProliferación celularQueratinasQueratinocitosRegulación hacia arribaSupervivencia celularÁcidos10.3389/fbioe.2022.876734PMC9159156https://www.frontiersin.org/articles/10.3389/fbioe.2022.876734/pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159156/pdf