López-Longarela, BarbaraMorrison, Emma ETranter, John DChahman-Vos, LianneLéonard, Jean-FrançoisGautier, Jean-CharlesLaurent, SébastienLartigau, AudeBoitier, EricSautier, LucileCarmona-Saez, PedroMartorell-Marugan, JordiMellanby, Richard JPernagallo, SalvatoreIlyine, HughRissin, David MDuffy, David CDear, James WDíaz-Mochón, Juan J2023-02-082023-02-082020-01-27http://hdl.handle.net/10668/14958Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10 μL of serum or plasma without any extraction steps, with a limit of detection of 1.32 pM that enabled the identification of DILI. Testing of 192 human serum samples showed that DCL accurately identified patients at risk of DILI after acetaminophen overdose (area under ROC curve 0.98 (95% CI; 0.96-1), PenAcetaminophenAdolescentAdultAnimalsBiomarkersChemical and Drug Induced Liver InjuryDogsHepatocytesHumansMaleMicroRNAsRatsYoung Adultresearch article31939284open access10.1021/acs.analchem.9b054491520-6882http://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81