O'Valle, FranciscoPeregrina, MagdalenaCrespo-Lora, VicenteGalindo-Moreno, PabloRoman, MariaPadial-Molina, MiguelMesa, FranciscoAneiros-Fernandez, JoseAguilar, DavidGonzalez-Rey, ElenaDelgado, MarioHernandez-Cortes, Pedro2015-12-022015-12-022015O'Valle F, Peregrina M, Crespo-Lora V, Galindo-Moreno P, Roman M, Padial-Molina M, et al. Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis. Biomed Res Int. 2015:681456http://hdl.handle.net/10668/2097Journal Article;Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein. Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1. Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (P < 0.001). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions (P < 0.0001) and was related to the severity of the lesions (Spearman's rho = 0.775, P = 0.0001). Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.enModelos teóricosArtritis experimentalColágeno tipo IIRatonesCélulas madre adultasInmunizaciónInmunoglobulina gArticulacionesAnimalesMasculinoFactor de necrosis tumoral alfaReceptores del factor de necrosis tumoralMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, TheoreticalMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal::Disease Models, Animal::Arthritis, ExperimentalMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::CollagenMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Anatomy::Cells::Stem Cells::Adult Stem CellsMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen::Fibrillar Collagens::Collagen Type IIMedical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Services::Preventive Health Services::Primary Prevention::ImmunizationMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin GMedical Subject Headings::Anatomy::Musculoskeletal System::Skeleton::JointsMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Check Tags::MaleMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Tumor Necrosis Factors::Tumor Necrosis Factor-alphaMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Tumor Necrosis Factorresearch article26064941open access10.1155/2015/6814562314-6141PMC4433648