García-Fontana, CristinaRodriguez-Sanchez, AlejandroMuñoz-Palazon, BarbaraGonzalez-Martinez, AlejandroVela-Cano, MariaGonzalez-Lopez, Jesus2022-02-252022-02-252020-08-18García-Fontana C, Rodriguez-Sanchez A, Muñoz-Palazon B, Gonzalez-Martinez A, Vela-Cano M, Gonzalez-Lopez J. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water. 2020 Aug 18;12(8):2316.http://hdl.handle.net/10668/3454In wastewater treatment plants, most microbial characterization has focused on bacterial, archaeal, and fungal populations. Due to the difficult isolation, quantification, and identification of viruses, only a limited number of virome studies associated with wastewater treatment plants have been carried out. However, the virus populations play an important role in the microbial dynamics in wastewater treatment systems and the biosafety of effluents. In this work, the viral members present in influent wastewater, mixed liquor (aerobic bioreactor), excess sludge, and effluent water of a conventional activated sludge system for the treatment of urban wastewater were identified. Viral members were observed by transmission electron microscopy and studied through next-generation sequencing studies. The results showed the dominance of bacteriophages in the viral community in all samples, with the dominant viral phylotype classified as Escherichia coli O157 typing phage 7. Moreover, different human viruses, such as Cynomolgus cytomegalovirus and Gammaherpesvirus, were also detected.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Next-generation sequencingSewage viromeViral distributionVirusTransmission electron microscopyWastewater treatment plantSecuenciación de nueva generaciónMicroscopía electrónica de transmisiónPlantas de tratamiento de aguas residualesAndalucíaAndalusianEspañaSpainMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy::Microscopy, Electron::Microscopy, Electron, TransmissionMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::High-Throughput Nucleotide SequencingMedical Subject Headings::Health Care::Environment and Public Health::Public Health::Public Health Practice::Communicable Disease Control::Sanitation::Sanitary Engineering::Waste Management::Water PurificationMedical Subject Headings::Organisms::VirusesMedical Subject Headings::Geographical Locations::Geographic Locations::Europe::SpainMedical Subject Headings::Chemicals and Drugs::Complex Mixtures::Waste Products::Waste Water::SewageMedical Subject Headings::Health Care::Environment and Public Health::Public Health::Environmental Pollution::Waste Products::Waste WaterMedical Subject Headings::Organisms::Viruses::Bacteriophages::MycobacteriophagesMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Organisms::Viruses::DNA Viruses::Herpesviridae::Betaherpesvirinae::CytomegalovirusMedical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Escherichia::Escherichia coli::Shiga-Toxigenic Escherichia coli::Enterohemorrhagic Escherichia coli::Escherichia coli O157Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Containment of BiohazardsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::BioreactorsMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Hydroxides::Waterresearch articleopen access10.3390/w120823162073-4441