Oluwabusola, Emmanuel T.Adebisi, Olusoji O.Reyes, FernandoAcquah, Kojo S.De la Cruz, MercedesMweetwa, Larry L.Rajakulendran, Joy E.Warner, Digby F.Hai, DengEbel, RainerJaspars, Marcel2023-02-122023-02-122021-09-141860-5397http://hdl.handle.net/10668/19338Five new phenolic siderophores 1-5 were isolated from the organic extract of a culture broth in a modified SGG medium of Pseu-domonas sp. UIAU-6B, obtained from sediments collected from the Oyun river in North Central Nigeria. The structure of the new compounds, pseudomonin A-C (1-3) and pseudomobactin A and B (4 and 5) isolated alongside two known compounds, pseudomonine (6) and salicylic acid (7), were elucidated based on high-resolution mass spectrometry, 1D and 2D NMR analyses. The absolute configuration of the threonine residue in compounds 1-5 was determined by Marfey analysis. The antimicrobial eval-uation of compound 4 exhibited the most potent activity against vancomycin-sensitive Enterococcus faecium VS144754, followed by 3 and 5, with MIC values ranging from 8 to 32 mu g/mL. Compounds 2 and 3 exhibited moderate activity against Mycobacterium tuberculosis H37Rv, with MIC values of 7.8 and 15.6 mu g/mL, respectively. Plausible biosynthetic hypotheses toward the new com-pounds 1-5 were proposed.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Mycobacterium tuberculosisPhenolic siderophorespseudomoninePseudomonas spvancomycin-sensitive Enterococcus faeciumNatural-productsAntibacterial activityAntifungal-activityGenome sequenceSoil bacteriumSalicylic-acidIronBiosynthesisAccumulationRhizosphereresearch articleopen access10.3762/bjoc.17.156https://www.beilstein-journals.org/bjoc/content/pdf/1860-5397-17-156.pdf697024900001