Hernaez, AlvaroJaramillo, SaraGarcia-Borrego, AránzazuEspejo-Calvo, Juan AntonioCovas, Maria-IsabelBlanchart, Gemmade la Torre, RafaelCarrasco-Pancorbo, AlegríaMesa, Maria DoloresFernandez-Prior, Maria AfricaCastañer, OlgaFito, Montserrat2023-02-092023-02-092021-01-26Hernáez Á, Jaramillo S, García-Borrego A, Espejo-Calvo JA, Covas MI, Blanchart G, et al. From Green Technology to Functional Olive Oils: Assessing the Best Combination of Olive Tree-Related Extracts with Complementary Bioactivities. Antioxidants (Basel). 2021 Jan 30;10(2):202.2076-3921http://hdl.handle.net/10668/17160Our aim was to assess the combination of olive tree-related extracts with the most favorable profile of in vitro bioactive properties. We tested the antioxidant (increment of low-density lipoprotein resistance against oxidation), vasoactive (promotion of nitric oxide release and decrease of endothelin-1 production in human umbilical vein endothelial cells), anti-inflammatory (decrease of the endothelial production of vascular cell adhesion molecule-1), and antithrombotic (reduction of the endothelial release of plasminogen activator inhibitor-1) capacities of six phenolic extracts and three triterpenic acid solutions (Ps and Ts, respectively). We tested extracts alone and in combination, at nutritional (Ps: 0.05-0.5 μmol/L; Ts: 0.001-0.1 μmol/L) and nutraceutical doses (Ps: 1-10 μmol/L; Ts: 0.25-10 μmol/L). The combination of Ps rich in 3,4-dihydroxyphenylglycol (76%, P2), hydroxytyrosol (95%, P3), and oleuropein (70%, P4) (final nutritional concentration: 0.15 μmol/L; final nutraceutical concentration: 3 μmol/L) was the best in order to prepare functional products and nutraceuticals with cardioprotective properties, despite the fact that the isolated extract with the greatest in vitro properties was P5 (75% oleocanthal), suggesting a potential synergistic effect among different olive components.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/3,4-dihydroxyphenylglycolbioactivityfunctional olive oilhydroxytyrosololeocanthaloleuropeinolive treephenolic compoundtriterpenesAntioxidantsOleaEndothelin-1Nitric OxideVascular Cell Adhesion Molecule-1Fibrinolytic AgentsHuman Umbilical Vein Endothelial Cells3,4-dihydroxyphenylethanolLipoproteins, LDLDietary SupplementsPlant ExtractsAnti-Inflammatory AgentsPlasminogen Inactivatorsresearch article33573339open accessAntiinflamatoriosAntioxidantesCélulas endoteliales de la vena umbilical humanaEndotelina-1Extractos vegetalesFibrinolíticosInactivadores plasminogénicosLipoproteínas LDLMolécula 1 de adhesión celular vascularOleaSuplementos dietéticosÓxido Nítrico10.3390/antiox10020202PMC7912092https://www.mdpi.com/2076-3921/10/2/202/pdf?version=1612332179https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912092/pdf