Lopez-Baltanas, RodrigoEncarnacion Rodriguez-Ortiz, MariaCanalejo, AntonioDiaz-Tocados, Juan MHerencia, CarmenLeiva-Cepas, FernandoTorres-Peña, Jose DOrtiz-Morales, AnaMuñoz-Castañeda, Juan RafaelRodriguez, MarianoAlmaden, Yolanda2023-02-092023-02-092021-03-29López-Baltanás R, Encarnación Rodríguez-Ortiz M, Canalejo A, Díaz-Tocados JM, Herencia C, Leiva-Cepas F, et al. Magnesium supplementation reduces inflammation in rats with induced chronic kidney disease. Eur J Clin Invest. 2021 Aug;51(8):e13561http://hdl.handle.net/10668/17599Inflammation is a common feature in chronic kidney disease (CKD) that appears specifically associated with cardiovascular derangements in CKD patients. Observational studies have revealed a link between low Mg levels and inflammation. In this study, we hypothesize that Mg might have a modulatory effect on the inflammation induced under the uraemic milieu. In vivo studies were performed in a 5/6 nephrectomized rat model of CKD. Furthermore, a possible direct effect of Mg was addressed through in vitro studies with vascular smooth muscle cells (VSMCs). Uraemic rats fed a normal (0.1%) Mg diet showed a systemic inflammatory response evidenced by the elevation in plasma of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6, and GPx activity, a marker of oxidative stress. Importantly, an increased expression of these cytokines in the aortic tissue was also observed. In contrast, a dietary Mg supplementation (0.6%) greatly prevented the oxidative stress and the pro-inflammatory response. In vitro, in VSMCs cultured in a pro-inflammatory high phosphate medium, incubation with Mg 1.6 mM inhibited the increase in the production of ROS, the rise in the expression of TNF-α, IL-1β, IL-6 and IL-8 and the activation of NF-κB signalling that was observed in cells incubated with a normal (0.8 mM) Mg. Mg supplementation reduced inflammation associated with CKD, exerting a direct effect on vascular cells. These findings support a possible beneficial effect of Mg supplementation along the clinical management of CKD patients.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Chronic kidney diseaseInflammationMagnesiumPhosphateUraemiaVascular smooth muscle cellsAnimalsCells, culturedCytokinesDietary supplementsInflammationMagnesiumMaleMyocytes, smooth muscleOxidative stressRatsRats, WistarReactive oxygen speciesRenal insufficiency, chronicSignal transductionresearch article33870500open accessCitocinasCélulas cultivadasEspecies reactivas de oxígenoEstrés oxidativoInflamaciónInsuficiencia renal crónicaMagnesioSuplementos dietéticosTransducción de señal10.1111/eci.135611365-2362http://rabida.uhu.es/dspace/bitstream/10272/19854/4/Magnesium.pdf