Rodríguez-Mateo, CristinaTorres, BelénGutiérrez, GabrielPintor-Toro, José A2023-01-252023-01-252017-02-10http://hdl.handle.net/10668/10857Long non-coding RNAs (lncRNAs) are a class of regulatory genes that participate in a wide range of biological processes, including proliferation, differentiation and development, as well as in a broad spectrum of diseases. Although the role of lncRNAs in TGF-β-induced epithelial-to-mesenchymal transition (EMT) has been well established, little is known about the role of lncRNAs as immediate-early regulators of EMT. Here lnc-Spry1 is identified as an immediate-early regulator of EMT that is downregulated by TGF-β. It is also found that knockdown of lnc-Spry1 promotes a mesenchymal-like phenotype and results in increased cell migration and invasion. In addition, it is shown that lnc-Spry1 depletion preferentially affects the expression of TGF-β-regulated gene targets. Moreover, lnc-Spry1 associates with U2AF65 splicing factor, suggesting a role in alternative splicing. Depletion of lnc-Spry1 induces, as TGF-β, isoform switching of fibroblast growth factor receptors, resulting in FGF-2-sensitive cells. Taken together, these results show that lnc-Spry1 could act as an early mediator of TGF-β signaling and reveal different roles for a lncRNA in modulating transcriptional and posttranscriptional gene expression.enAdaptor Proteins, Signal TransducingAnimalsCell LineCell MovementCell ProliferationEpithelial CellsEpithelial-Mesenchymal TransitionFemaleFibroblast Growth Factor 2Mammary Glands, AnimalMembrane ProteinsMiceMice, Inbred C57BLMutationPhosphoproteinsRNA Processing, Post-TranscriptionalRNA, Long NoncodingReceptors, Fibroblast Growth FactorSignal TransductionSplicing Factor U2AFTranscription, GeneticTransforming Growth Factor betaresearch article28186499open access10.1038/cdd.2017.91476-5403PMC5423121https://www.nature.com/articles/cdd20179.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423121/pdf