Llorente-Cantarero, Francisco JesúsAguilar-Gómez, Francisco JavierLeis, RosauraBueno, GloriaRupérez, Azahara I.Anguita-Ruiz, AugustoVázquez-Cobela, RocíoMesa, María DoloresMoreno, Luis A.Gil, ÁngelAguilera, Concepción MaríaGil-Campos, Mercedes2022-03-302022-03-302021-02-20Llorente-Cantarero FJ, Aguilar-Gomez FJ, Leis R, Bueno G, Ruperez AI, Anguita-Ruiz A, et al. Relationship between Physical Activity, Oxidative Stress, and Total Plasma Antioxidant Capacity in Spanish Children from the GENOBOX Study. Antioxidants. 2021;10(2):320http://hdl.handle.net/10668/3506The World Health Organization has recommended performing at least 60 min a day of moderate-to-vigorous physical activity (MVPA) and reducing sedentarism in children and adolescents to offer significant health benefits and mitigate health risks. Physical fitness and sports practice seem to improve oxidative stress (OS) status during childhood. However, to our knowledge, there are no data regarding the influence of objectively-measured physical activity (PA) and sedentarism on OS status in children and adolescents. The present study aimed to evaluate the influence of moderate and vigorous PA and sedentarism on OS and plasma total antioxidant capacity (TAC) in a selected Spanish population of 216 children and adolescents from the GENOBOX study. PA (light, moderate, and vigorous) and sedentarism (i.e., sedentary time (ST)) were measured by accelerometry. A Physical Activity-Sedentarism Score (PASS) was developed integrating moderate and vigorous PA and ST levels. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and isoprostane F2α (F2-IsoPs), as markers of OS, were determined by ELISA; and TAC was estimated by colorimetry using an antioxidant kit. A higher PASS was associated with lower plasma TAC and urinary 8-OHdG and F2-IsoPs, showing a better redox profile. Reduced OS markers (8-OHdG and F2-IsoPs) in children with higher PASS may diminish the need of maintaining high concentrations of antioxidants in plasma during rest to achieve redox homeostasis.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Physical activityAccelerometryOxidative stressPlasma total antioxidant capacity8-hydroxy-2′-deoxyguanosineIsoprostane F2αEjercicio físicoAcelerometríaEstrés oxidativo8-Hidroxi-2'-DesoxicoguanosinaMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Protective Agents::AntioxidantsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Titrimetry::ColorimetryMedical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Physical FitnessMedical Subject Headings::Health Care::Health Care Economics and Organizations::Organizations::International Agencies::United Nations::World Health OrganizationMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative StressMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assayresearch article33672676open access10.3390/antiox100203202076-3921PMC7924393