Ruiz-Malagon, Antonio JesusRodriguez-Sojo, Maria JesusHidalgo-Garcia, LauraMolina-Tijeras, Jose AlbertoGarcia, FedericoPischel, IvoRomero, MiguelDuarte, JuanDiez-Echave, PatriciaRodriguez-Cabezas, Maria ElenaRodriguez-Nogales, AlbaGalvez, Julio2023-05-032023-05-032022-05-24Ruiz-Malagón AJ, Rodríguez-Sojo MJ, Hidalgo-García L, Molina-Tijeras JA, García F, et al. The Antioxidant Activity of Thymus serpyllum Extract Protects against the Inflammatory State and Modulates Gut Dysbiosis in Diet-Induced Obesity in Mice. Antioxidants (Basel). 2022 May 28;11(6):1073.2076-3921http://hdl.handle.net/10668/20782Nowadays, there is an increasing interest in alternative therapies in the treatment of metabolic syndrome that combine efficacy and safety profiles. Therefore, this study aimed to evaluate the effect of an extract of Thymus serpyllum, containing rosmarinic acid, on high-fat diet (HFD)-induced obesity mice, highlighting the impact of its antioxidant activity on the inflammatory status and gut dysbiosis. The extract was administered daily (50, 100 and 150 mg/kg) in HFD-fed mice. The treatment reduced body weight gain, glucose and lipid metabolic profiles. Moreover, the extract ameliorated the inflammatory status, with the c-Jun N-terminal kinases (JUNK) pathway being involved, and showed a significant antioxidant effect by the reduction of radical scavenging activity and the mitigation of lipid peroxidation. Moreover, the extract was able to modulate the altered gut microbiota, restoring microbial richness and diversity, and augmenting the counts of short-chain fatty acid producing bacteria, which have been associated with the maintenance of gut permeability and weight regulation. In conclusion, the antioxidant activity of Thymus serpyllum extract displayed a positive impact on obesity and its metabolic alterations, also reducing systemic inflammation. These effects may be mediated by modulation of the gut microbiota.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Thymus serpyllumanti-inflammatoryantioxidantgut microbiotahigh fat dietobesityMetabolic SyndromeAntioxidantsRosmarinic AcidDiet, High-FatJNK Mitogen-Activated Protein KinasesGastrointestinal MicrobiomeDysbiosisObesityWeight GainComplementary TherapiesFatty Acids, VolatileBacteriaPermeabilityresearch article35739969open accessAntioxidantesAumento de pesoBacteriasDieta alta en grasaDisbiosisInflamaciónMetabolomaMicrobioma gastrointestinalObesidadPermeabilidadProteínas quinasas JNK activadas por mitógenosSíndrome metabólico10.3390/antiox11061073PMC9219752https://www.mdpi.com/2076-3921/11/6/1073/pdf?version=1653728362https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219752/pdf