Amador-Alvarez, AidaGomez-Muñoz, María ARodriguez-Prieto, IsmaelPardal, RicardoVega, Francisco M2023-05-032023-05-032022-03-23Amador-Álvarez A, Gómez-Muñoz MA, Rodríguez-Prieto I, Pardal R, Vega FM. A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines. STAR Protoc. 2022 Mar 23;3(2):101260.http://hdl.handle.net/10668/22542The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/CancerCell BiologyCell cultureCell isolationFlow Cytometry/Mass CytometrySingle CellStem CellsCell Culture TechniquesCell LineCell SeparationCells, CulturedHumansNeuroblastomaresearch article35345594open accessCélulasNeoplasiasNeuroblastomaMicroscopía electrónica de transmisión de rastreoCulturaBiomarcadoresTécnicas In VitroPoblaciónTécnicas de cultivo de célula10.1016/j.xpro.2022.1012602666-1667PMC8956940https://doi.org/10.1016/j.xpro.2022.101260https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956940/pdf