Bruzelius, AndreasHidalgo, IsabelBoza-Serrano, AntonioHjelmér, Anna-GiorgiaTison, AmelieDeierborg, TomasBengzon, JohanRamos-Moreno, Tania2023-02-092023-02-092020-12-09http://hdl.handle.net/10668/16762Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow-derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia-like cells to use in cell-based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR-MP). STR-MP single-cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial-specific genes present in development and CNS pathologies. STR-MPs can be expanded and generate microglia-like cells in vitro, which we name stromal microglia (STR-M). STR-M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia-like precursors that can be used in patient-centered fast derivative approaches.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/bone marrowcommon myeloid progenitorhuman bone marrowmicrogliamicroglia-like cell in vitro modelmicroglial precursorpluripotent stem cellprimitive myeloid progenitorBone MarrowCD11b AntigenCentral Nervous SystemHumansLeukocyte Common AntigensMicrogliaStem Cellsresearch article33295698open access10.1002/sctm.20-01272157-6580PMC7980218https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/sctm.20-0127https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980218/pdf