Lopez-Caraballo, LidiaMartorell-Marugan, JordiCarmona-Saez, PedroGonzalez-Muñoz, Elena2022-04-202022-04-202020-08-21Lopez-Caraballo L, Martorell-Marugan J, Carmona-Saez P, Gonzalez-Muñoz E. Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming. iScience. 2020 Aug 21;23(8):101376http://hdl.handle.net/10668/3545Cell reprogramming has revolutionized cell and regenerative biology field. However, human iPS derivation remains inefficient and variable. A better knowledge of molecular processes and the rationale underlying the importance of somatic cell origin is crucial to uncover reprogramming mechanisms. Here, we analyze the molecular profile of different human somatic cell types. We show menstrual blood-derived stromal cells (MnSCs) have a distinct, reprogramming prone, profile, and we identify SOX15 from their oocyte-related signature as a prominent responsible candidate. SOX15 orchestrates an efficient oocyte-based reprogramming combination when overexpressed with the also oocyte-enriched histone chaperone ASF1A and OCT4 and, through specific mechanism, generates iPSCs with distinguishable pluripotent state that further present higher differentiation capacity than canonical iPSCs. Our work supports the presence of different pluripotency states in reprogramming and the importance of using metaphase-II oocyte and MnSCs information to provide alternative reprogramming combinations and, importantly, to improve and understand pluripotency acquisition.enAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Stromal cellsMenstruationBlood cellsCellular reprogrammingOocytesCélulas del estromaMenstruaciónCélulas sanguíneasReprogramación celularOocitosMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::MetaphaseMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell DifferentiationMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Molecular Chaperones::Histone ChaperonesMedical Subject Headings::Anatomy::Cells::Germ Cells::Ovum::OocytesMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal CellsMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::BiologyMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Menstrual Cycle::Menstruationresearch article32738616Acceso abierto10.1016/j.isci.2020.1013762589-0042PMC7394916