Garcia-Moreno, AdrianCarmona-Saez, Pedro2022-07-222022-07-222020-08-28Garcia-Moreno A, Carmona-Saez P. Computational Methods and Software Tools for Functional Analysis of miRNA Data. Biomolecules. 2020 Aug 28;10(9):1252http://hdl.handle.net/10668/3812miRNAs are important regulators of gene expression that play a key role in many biological processes. High-throughput techniques allow researchers to discover and characterize large sets of miRNAs, and enrichment analysis tools are becoming increasingly important in decoding which miRNAs are implicated in biological processes. Enrichment analysis of miRNA targets is the standard technique for functional analysis, but this approach carries limitations and bias; alternatives are currently being proposed, based on direct and curated annotations. In this review, we describe the two workflows of miRNAs enrichment analysis, based on target gene or miRNA annotations, highlighting statistical tests, software tools, up-to-date databases, and functional annotations resources in the study of metazoan miRNAs.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Functional analysisMiRNANcRNADatabasesEnrichmentToolsMicroARNsARN largo no codificanteBases de datos genéticasProgramas informáticosMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational BiologyMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Data Interpretation, StatisticalMedical Subject Headings::Information Science::Information Science::Medical Informatics::Medical Informatics Applications::Information Systems::Databases as Topic::Databases, Factual::Databases, GeneticMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::MicroRNAsMedical Subject Headings::Information Science::Information Science::Information Services::Documentation::Molecular Sequence Data::Molecular Sequence AnnotationMedical Subject Headings::Information Science::Information Science::Computing Methodologies::Softwarereview article32872205Acceso abierto10.3390/biom100912522218-273XPMC7563698