Murtazina, AlimaRuiz Alcala, GloriaJimenez-Martinez, YaizaMarchal, Juan AntonioTarabayeva, AnelBitanova, ElmiraMcDougall, GordonBishimbayeva, NaziraBoulaiz, Houria2023-05-032023-05-032022-05-17Murtazina A, Ruiz Alcala G, Jimenez-Martinez Y, Marchal JA, Tarabayeva A, Bitanova E, et al. Potential of Polysaccharides Derived from Wheat Cell Culture. Pharmaceutics. 2022 May 20;14(5):1100.1999-4923http://hdl.handle.net/10668/21549There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/apoptosiscolon cancer cellscytochrome cdifferentiationelectron microscopyinhibition of proliferationplant polysaccharideswheat cell cultureTriticumCytochromes cbcl-2-Associated X ProteinCateninsMicroscopy, Electron, ScanningPlants, ToxicApoptosisMitochondriaCell Cycle CheckpointsColonic NeoplasmsG1 PhaseCell ProliferationCell DifferentiationCaspasesCell Culture TechniquesCell Lineresearch article35631686open accessApoptosisCaspasasCateninasCitocromos cDiferenciación celularFase G1Línea celularMicroscopía dlectrónica de rastreoMitocondriasNeoplasias del colonPlantas tóxicasProliferación celularProteína X asociada a bcl-2Puntos de control del ciclo celular10.3390/pharmaceutics14051100PMC9147229https://www.mdpi.com/1999-4923/14/5/1100/pdf?version=1653470526https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147229/pdf