Ruiz-Ojeda, Francisco JavierIris Ruperez, AzaharaGomez-Llorente, CarolinaGil, AngelMaria Aguilera, Concepcion2023-02-122023-02-122016-06-24Ruiz-Ojeda FJ, Rupérez AI, Gomez-Llorente C, Gil A, Aguilera CM. Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review. Int J Mol Sci. 2016 Jun 30;17(7):1040.http://hdl.handle.net/10668/19267Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/adipocytesbeige cellsbrown adipose tissuecell culture techniquescell differentiationin vitro techniquesobesitywhite adipose tissueBrown adipose-tissueInhibits adipocyte differentiationMesenchymal stem-cellsMorphogenetic protein 4Human pre-adipocytesHuman preadipocytesWhite fatPorcine preadipocytesGene-expression3t3-l1 cellsHumansAnimalsAdipogenesisCoculture TechniquesPrevalenceAdipose TissueAdipocytesCell Culture TechniquesObesityMolecular BiologyBiologyreview27376273open accessAdipocitosAdipogénesisAnimalesBiologíaBiología molecularHumanosObesidadPrevalenciaTejido adiposoTécnicas de cocultivoTécnicas de cultivo de célula10.3390/ijms170710401422-0067https://www.mdpi.com/1422-0067/17/7/1040/pdf?version=1467285286381500900055