Clemente, Luis FelipeHernáez, María LuisaRamos-Fernández, AntonioLigero, GertrudisGil, ConchaCorrales, Fernando JoséMarcilla, Miguel2023-01-252023-01-252018-07-06http://hdl.handle.net/10668/12675Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.enAdipose TissueHumansHyaluronan SynthasesMesenchymal Stem CellsPeptidesProteomeUmbilical Cordresearch article29974749open access10.1021/acs.jproteome.8b003841535-3907https://eprints.ucm.es/55669/1/Clemente%20F%20et%20al%20para%20el%20repositorio.pdf