Alvarez-Cubero, M JSantiago, OMartínez-Labarga, CMartínez-García, BMarrero-Díaz, RRubio-Roldan, APérez-Gutiérrez, A MCarmona-Saez, PLorente, J AMartinez-Gonzalez, L J2023-01-252023-01-252018-06-21http://hdl.handle.net/10668/12631This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Cells, CulturedChromosomes, Human, YFlow CytometryHigh-Throughput Nucleotide SequencingHumansLaser Capture MicrodissectionMaleSequence Analysis, DNAresearch article29930304open access10.1038/s41598-018-27819-x2045-2322PMC6013464https://www.nature.com/articles/s41598-018-27819-x.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013464/pdf