Serrano-Benítez, AlmudenaCortés-Ledesma, FelipeRuiz, Jose F.2022-07-062022-07-062020-01-10Serrano-Benítez A, Cortés-Ledesma F, Ruiz JF. "An End to a Means": How DNA-End Structure Shapes the Double-Strand Break Repair Process. Front Mol Biosci. 2020 Jan 10;6:153http://hdl.handle.net/10668/3746Endogenously-arising DNA double-strand breaks (DSBs) rarely harbor canonical 5'-phosphate, 3'-hydroxyl moieties at the ends, which are, regardless of the pathway used, ultimately required for their repair. Cells are therefore endowed with a wide variety of enzymes that can deal with these chemical and structural variations and guarantee the formation of ligatable termini. An important distinction is whether the ends are directly "unblocked" by specific enzymatic activities without affecting the integrity of the DNA molecule and its sequence, or whether they are "processed" by unspecific nucleases that remove nucleotides from the termini. DNA end structure and configuration, therefore, shape the repair process, its requirements, and, importantly, its final outcome. Thus, the molecular mechanisms that coordinate and integrate the cellular response to blocked DSBs, although still largely unexplored, can be particularly relevant for maintaining genome integrity and avoiding malignant transformation and cancer.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/DNA double strand break (DSB)Non-homologous DNA end joiningATMDNA-PK catalytic subunitGenome instabilityRoturas del ADN de doble cadenaReparación del ADN por unión de extremidadesProteínas de la ataxia telangiectasia mutadaProteína quinasa activada por ADNADNMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Damage::DNA Breaks::DNA Breaks, Double-StrandedMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Repair::DNA End-Joining RepairMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Serine-Threonine Kinases::Ataxia Telangiectasia Mutated ProteinsMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Serine-Threonine Kinases::DNA-Activated Protein KinaseMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Base SequenceMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNAreview article31998749Acceso abierto10.3389/fmolb.2019.001532296-889XPMC6965357