Alvarez-Mercado, Ana IPlaza-Diaz, Juliode Almagro, M CristinaGil, AngelMoreno-Muñoz, Jose AntonioFontana, Luis2023-05-032023-05-032022-09-06Álvarez-Mercado AI, Plaza-Díaz J, de Almagro MC, Gil Á, Moreno-Muñoz JA, Fontana L. Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515. Int J Mol Sci. 2022 Sep 16;23(18):10813.http://hdl.handle.net/10668/21196Previous works have described the activity of Bifidobacterium longum subsp. infantis CECT 7210 (also commercially named B. infantis IM-1®) against rotavirus in mice and intestinal pathogens in piglets, as well as its diarrhea-reducing effect on healthy term infants. In the present work, we focused on the intestinal immunomodulatory effects of B. infantis IM-1® and for this purpose we used the epithelial cell line isolated from colorectal adenocarcinoma Caco-2 and a co-culture system of human dendritic cells (DCs) from peripheral blood together with Caco-2 cells. Single Caco-2 cultures and Caco-2: DC co-cultures were incubated with B. infantis IM-1® or its supernatant either in the presence or absence of Escherichia coli CECT 515. The B. infantis IM-1® supernatant exerted a protective effect against the cytotoxicity caused by Escherichia coli CECT 515 on single cultures of Caco-2 cells as viability reached the values of untreated cells. B. infantis IM-1® and its supernatant also decreased the secretion of pro-inflammatory cytokines by Caco-2 cells and the co-cultures incubated in the presence of E. coli CECT 515, with the response being more modest in the latter, which suggests that DCs modulate the activity of Caco-2 cells. Overall, the results obtained point to the immunomodulatory activity of this probiotic strain, which might underlie its previously reported beneficial effects.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/B. infantis IM-1®Bifidobacterium longum subsp. infantis CECT 7210Caco-2 cellsEscherichia colico-culturescytokinesdendritic cellsprobioticsAnimalsBifidobacteriumBifidobacterium longum subspecies infantisCaco-2 CellsCytokinesEscherichia coliEscherichia coli InfectionsHumansInfantMiceProbioticsSwineresearch article36142723open accessBifidobacteriumAnimalesBifidobacterium longum subspecies infantisCitocinasCélulas CACO-2Escherichia coliInfecciones por Escherichia coliLactantePorcinosProbióticos10.3390/ijms2318108131422-0067PMC9503999https://www.mdpi.com/1422-0067/23/18/10813/pdf?version=1663323463https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503999/pdf