Parra, DiegoPeña-Monje, AlejandroCoronado-Álvarez, Nieves MaríaHernández-Quero, JoséParra-Ruiz, Jorge2016-10-212016-10-212015-10-30Parra D, Peña-Monje A, Coronado-Álvarez NM, Hernández-Quero J, Parra-Ruiz J. In vitro efficacy of daptomycin and teicoplanin combined with ethanol, clarithromycin or gentamicin as catheter lock solutions. BMC Microbiol. 2015.; 15:245http://hdl.handle.net/10668/2479Journal Article;BACKGROUND Despite widespread use, optimum choice of antimicrobial agents, concentrations, combinations and exposure times have not been determined for antibiotic lock technique (ALT). Our objective was to evaluate the efficacy of different antibiotic combinations using an in vitro model of catheter-related infection. Daptomycin (DAP) 5 mg/mL, teicoplanin (TEC) 5 mg/mL, both alone and combined with gentamicin (GM) 2.5 mg/mL, clarythromycin (CLA) 5 mg/mL or ethanol 35 % were evaluated against four clinical strains of methicillin resistant coagulase negative staphylococci. Lock solutions were renewed every 24 h. RESULTS After 72 h catheters were reincubated with culture media to investigate bacterial regrowth. All antibiotic combinations resulted in significant reductions (p < 0.05) of Log(10) cfu/mL at 72 h for both organisms compared with controls. DAP resulted in significant reductions of Log(10) for all organism versus TEC (p = 0.001). Only DAP reached the limit of detection at 72 h, however did not prevent regrowth after 24 h of ALT removal. DAP + Ethanol and TEC + ethanol eradicated biofilm at 72 h, but only DAP + ethanol (against all strains) and DAP + CLA (against two strains) prevented regrowth at 24 h after ALT removal. CONCLUSIONS Based on these data, ALT with DAP + ethanol and DAP + CLA should be explored in clinical trials.enCentral line associated bloodstream infectionsAntibiotic lock therapyBiofilmAntibacterianosCatéteresClaritromicinaDaptomicinaQuimioterapia combinadaEtanolBiofilmesGentamicinasHumanosTécnicas In vitroResistencia a la meticilinaPruebas de sensibilidad microbianaTeicoplaninaVancomicinaMedical Subject Headings::Phenomena and Processes::Microbiological Phenomena::BiofilmsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::CathetersMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Lactones::Macrolides::Erythromycin::ClarithromycinMedical Subject Headings::Chemicals and Drugs::Polycyclic Compounds::Macrocyclic Compounds::Peptides, Cyclic::DaptomycinMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Drug Therapy::Drug Therapy, CombinationMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Alcohols::EthanolMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycosides::Aminoglycosides::GentamicinsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Drug Resistance, Microbial::Drug Resistance, Bacterial::beta-Lactam Resistance::Penicillin Resistance::Methicillin ResistanceMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity TestsMedical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus epidermidisMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycoconjugates::Glycopeptides::TeicoplaninMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycoconjugates::Glycopeptides::VancomycinMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial AgentsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::In Vitro Techniquesresearch article26518881open access10.1186/s12866-015-0585-31471-2180PMC4628264