Ortega-Sáenz, PatriciaPascual, AlbertoGómez-Díaz, RaquelLópez-Barneo, José2014-09-022014-09-022006-10Ortega-Sáenz P, Pascual A, Gómez-Díaz R, López-Barneo J. Acute oxygen sensing in heme oxygenase-2 null mice. J Gen Physiol. 2006; 128(4):405-10022-1295http://hdl.handle.net/10668/1703Journal Article; Research Support, Non-U.S. Gov't;. A Pascual is an investigator of the “Ramón y Cajal” program. J. López-Barneo received the “Ayuda a la Investigación 2000” of the Juan March FoundationHemeoxygenase-2 (HO-2) is an antioxidant enzyme that can modulate recombinant maxi-K(+) channels and has been proposed to be the acute O(2) sensor in the carotid body (CB). We have tested the physiological contribution of this enzyme to O(2) sensing using HO-2 null mice. HO-2 deficiency leads to a CB phenotype characterized by organ growth and alteration in the expression of stress-dependent genes, including the maxi-K(+) channel alpha-subunit. However, sensitivity to hypoxia of CB is remarkably similar in HO-2 null animals and their control littermates. Moreover, the response to hypoxia in mouse and rat CB cells was maintained after blockade of maxi-K(+) channels with iberiotoxin. Hypoxia responsiveness of the adrenal medulla (AM) (another acutely responding O(2)-sensitive organ) was also unaltered by HO-2 deficiency. Our data suggest that redox disregulation resulting from HO-2 deficiency affects maxi-K(+) channel gene expression but it does not alter the intrinsic O(2) sensitivity of CB or AM cells. Therefore, HO-2 is not a universally used acute O(2) sensor.enAnimalesAnimales Recién NacidosCalcioCuerpo CarotídeoCatecolaminasCélulas CromafinesCiclofilinasExpresión GénicaHemo Oxigenasa (Deciclizadora)Hemo-Oxigenasa 1Subunidades alfa de los Canales de Potasio de Gran Conductancia Activados por CalcioCanales de Potasio de Gran Conductancia Activados por el CalcioRatonesRatones Consanguíneos C57BLRatones NoqueadosTamaño de los ÓrganosOxígenoPresión ParcialTécnicas de Placa-ClampPéptidosBloqueadores de los Canales de PotasioRatasMédula SuprarrenalMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, NewbornMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Respiratory::AnoxiaMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Elements::Metals, Alkaline Earth::CalciumMedical Subject Headings::Anatomy::Nervous System::Neurons::Neurons, Afferent::Sensory Receptor Cells::Chemoreceptor Cells::Paraganglia, Nonchromaffin::Carotid BodyMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::CatecholaminesMedical Subject Headings::Anatomy::Cells::Chromaffin CellsMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Isomerases::cis-trans-Isomerases::Peptidylprolyl Isomerase::Immunophilins::CyclophilinsMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene ExpressionMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Heme Oxygenase (Decyclizing)Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Heme Oxygenase (Decyclizing)::Heme Oxygenase-1Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Membrane Transport Proteins::Ion Channels::Potassium Channels::Potassium Channels, Calcium-Activated::Large-Conductance Calcium-Activated Potassium ChannelsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred C57BLMedical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Genetically Modified::Mice, Transgenic::Mice, KnockoutMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ SizeMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Elements::Chalcogens::OxygenMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Pressure::Partial PressureMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Patch-Clamp TechniquesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::PeptidesMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Membrane Transport Modulators::Potassium Channel BlockersMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::RatsTyrosine 3-MonooxygenaseMedical Subject Headings::Anatomy::Endocrine System::Endocrine Glands::Adrenal Glands::Adrenal Medullaresearch article16966473open access10.1085/jgp.2006095911540-7748PMC2151578