Sanbonmatsu-Gamez, SaraPedrosa-Corral, IreneNavarro-Mari, Jose MariaPerez-Ruiz, Mercedes2023-02-092023-02-092021-07-19Sanbonmatsu-Gámez S, Pedrosa-Corral I, Navarro-Marí JM, Pérez-Ruiz M. Update in Diagnostics of Toscana Virus Infection in a Hyperendemic Region (Southern Spain). Viruses. 2021 Jul 23;13(8):1438http://hdl.handle.net/10668/18477The sandfly fever Toscana virus (TOSV, genus Phlebovirus, family Phenuiviridae) is endemic in Mediterranean countries. In Spain, phylogenetic studies of TOSV strains demonstrated that a genotype, different from the Italian, was circulating. This update reports 107 cases of TOSV neurological infection detected in Andalusia from 1988 to 2020, by viral culture, serology and/or RT-PCR. Most cases were located in Granada province, a hyperendemic region. TOSV neurological infection may be underdiagnosed since few laboratories include this virus in their portfolio. This work presents a reliable automated method, validated for the detection of the main viruses involved in acute meningitis and encephalitis, including the arboviruses TOSV and West Nile virus. This assay solves the need for multiple molecular platforms for different viruses and thus, improves the time to results for these syndromes, which require a rapid and efficient diagnostic approach.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/RT-PCRSpainToscana virusautomationencephalitismeningitisAutomation, LaboratoryBunyaviridae InfectionsCerebrospinal FluidEncephalitis, ArbovirusHumansMeningitis, ViralReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSandfly fever Naples virusSerologic Testsresearch article34452304open accessAutomatización de laboratoriosEncefalitis por arbovirusInfecciones por BunyaviridaeMeningitis viralPruebas serológicasReacción en cadena de la polimerasa de transcriptasa inversaReacción en Cadena en tiempo real de la polimerasaVirus de Nápoles de la fiebre de la mosca de los arenalesLíquido cefalorraquídeo10.3390/v130814381999-4915PMC8402649https://www.mdpi.com/1999-4915/13/8/1438/pdf?version=1627269217https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402649/pdf